Occurrence of aetokthonotoxin producer in natural samples-A PCR protocol for easy detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00583932" target="_blank" >RIV/60077344:_____/23:00583932 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/23:00579396 RIV/60076658:12310/23:43907136

Výsledek na webu

<a href="https://doi.org/10.1016/j.hal.2023.102425" target="_blank" >https://doi.org/10.1016/j.hal.2023.102425</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.hal.2023.102425" target="_blank" >10.1016/j.hal.2023.102425</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of aetokthonotoxin producer in natural samples-A PCR protocol for easy detection

Popis výsledku v původním jazyce

Cyanobacteria are well known producers of bioactive metabolites, including harmful substances. The recently discovered 'eagle killer' neurotoxin aetokthonotoxin (AETX) is produced by the epiphytic cyanobacterium Aetokthonos hydrillicola growing on invasive water thyme (Hydrilla verticillata). The biosynthetic gene cluster of AETX was previously identified from an Aetokthonos strain isolated from the J. Strom Thurmond Reservoir, Georgia, USA. Here, a PCR protocol for easy detection of AETX-producers in environmental samples of plant-cyanobacterium consortia was designed and tested. Three different loci of the AETX gene cluster were ampli-fied to confirm the genetic potential for AETX production, along with two variable types of rRNA ITS regions to confirm the homogeneity of the producer's taxonomic identity. In samples of Hydrilla from three Aetokthonos- positive reservoirs and one Aetokthonos-negative lake, the PCR of all four loci provided results congruent with the Aetokthonos presence/absence detected by light and fluorescence microscopy. The production of AETX in the Aetokthonos-positive samples was confirmed using LC-MS. Intriguingly, in J. Strom Thurmond Reservoir, recently Hydrilla free, an Aetokthonos-like cyanobacterium was found growing on American water-willow (Justicia americana). Those specimens were positive for all three aet markers but contained only minute amounts of AETX. The obtained genetic information (ITS rRNA sequence) and morphology of the novel Aetokthonos distinguished it from all the Hydrilla-hosted A. hydrillicola, likely at the species level. Our results suggest that the toxigenic Aetokthonos spp. can colonize a broader array of aquatic plants, however the level of accumulation of the toxin may be driven by host-specific interactions such as the locally hyper-accumulated bromide in Hydrilla.

Název v anglickém jazyce

Occurrence of aetokthonotoxin producer in natural samples-A PCR protocol for easy detection

Popis výsledku anglicky

Cyanobacteria are well known producers of bioactive metabolites, including harmful substances. The recently discovered 'eagle killer' neurotoxin aetokthonotoxin (AETX) is produced by the epiphytic cyanobacterium Aetokthonos hydrillicola growing on invasive water thyme (Hydrilla verticillata). The biosynthetic gene cluster of AETX was previously identified from an Aetokthonos strain isolated from the J. Strom Thurmond Reservoir, Georgia, USA. Here, a PCR protocol for easy detection of AETX-producers in environmental samples of plant-cyanobacterium consortia was designed and tested. Three different loci of the AETX gene cluster were ampli-fied to confirm the genetic potential for AETX production, along with two variable types of rRNA ITS regions to confirm the homogeneity of the producer's taxonomic identity. In samples of Hydrilla from three Aetokthonos- positive reservoirs and one Aetokthonos-negative lake, the PCR of all four loci provided results congruent with the Aetokthonos presence/absence detected by light and fluorescence microscopy. The production of AETX in the Aetokthonos-positive samples was confirmed using LC-MS. Intriguingly, in J. Strom Thurmond Reservoir, recently Hydrilla free, an Aetokthonos-like cyanobacterium was found growing on American water-willow (Justicia americana). Those specimens were positive for all three aet markers but contained only minute amounts of AETX. The obtained genetic information (ITS rRNA sequence) and morphology of the novel Aetokthonos distinguished it from all the Hydrilla-hosted A. hydrillicola, likely at the species level. Our results suggest that the toxigenic Aetokthonos spp. can colonize a broader array of aquatic plants, however the level of accumulation of the toxin may be driven by host-specific interactions such as the locally hyper-accumulated bromide in Hydrilla.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/GC19-21649J" target="_blank" >GC19-21649J: Lov na zabijáka orlů - výzkum cyanotoxinu způsobujícího ptačí vakuolární myelinopatii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Harmful Algae

ISSN

1568-9883

e-ISSN

1878-1470

Svazek periodika

125

Číslo periodika v rámci svazku

Mar

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

102425

Kód UT WoS článku

000967131700001

EID výsledku v databázi Scopus

2-s2.0-85151036490