High hymenopteran parasitoid infestation rates in Czech populations of the Euphydryas aurinia butterfly inferred using a new molecular marker

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00582054" target="_blank" >RIV/60077344:_____/24:00582054 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/24:43907980

Výsledek na webu

<a href="https://jhr.pensoft.net/article/113231/download/pdf/975500" target="_blank" >https://jhr.pensoft.net/article/113231/download/pdf/975500</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3897/jhr.97.113231" target="_blank" >10.3897/jhr.97.113231</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High hymenopteran parasitoid infestation rates in Czech populations of the Euphydryas aurinia butterfly inferred using a new molecular marker

Popis výsledku v původním jazyce

We apply a molecular approach to quantify the level of hymenopteran parasitoids infestation in the larvae of the marsh fritillary (Euphydryas aurinia), a declining butterfly species, in western Bohemia, Czech Republic, in two subsequent years. We used the novel primer HymR157 in combination with known universal 28SD1F to establish a PCR detection system which amplifies hymenopteran parasitoids, but not the lepidopteran host. In the 14 sampled E. aurinia colonies, the infestation rates per individuum were 33.3% and 40.2%, whereas per sampled larval colony, these were on average 38.5% (range 0–100) and 40.1% (0–78). The per-colony infestation rates correlated with the numbers of larval webs censused perncolony the year prior to sampling the parasitoids, pointing to a time lag in parasitoid infestation rates. The levels of the hymenopteran parasitoid prevalence are thus relatively high, supporting the importance of parasitoids for the population dynamics of the threatened host. The detection primers we developed can detect a range of hymenopteran parasitoids on other butterfly hosts.

Název v anglickém jazyce

High hymenopteran parasitoid infestation rates in Czech populations of the Euphydryas aurinia butterfly inferred using a new molecular marker

Popis výsledku anglicky

We apply a molecular approach to quantify the level of hymenopteran parasitoids infestation in the larvae of the marsh fritillary (Euphydryas aurinia), a declining butterfly species, in western Bohemia, Czech Republic, in two subsequent years. We used the novel primer HymR157 in combination with known universal 28SD1F to establish a PCR detection system which amplifies hymenopteran parasitoids, but not the lepidopteran host. In the 14 sampled E. aurinia colonies, the infestation rates per individuum were 33.3% and 40.2%, whereas per sampled larval colony, these were on average 38.5% (range 0–100) and 40.1% (0–78). The per-colony infestation rates correlated with the numbers of larval webs censused perncolony the year prior to sampling the parasitoids, pointing to a time lag in parasitoid infestation rates. The levels of the hymenopteran parasitoid prevalence are thus relatively high, supporting the importance of parasitoids for the population dynamics of the threatened host. The detection primers we developed can detect a range of hymenopteran parasitoids on other butterfly hosts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Hymenoptera Research

ISSN

1070-9428

e-ISSN

1314-2607

Svazek periodika

97

Číslo periodika v rámci svazku

JAN 29

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

14

Strana od-do

29-42

Kód UT WoS článku

001202310300001

EID výsledku v databázi Scopus

2-s2.0-85185508488