Different fates of metabolites and small variation in chemical composition characterise frass chemistry in a specialist caterpillar

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00582636" target="_blank" >RIV/60077344:_____/24:00582636 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/24:43907986

Výsledek na webu

<a href="https://resjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/phen.12429" target="_blank" >https://resjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/phen.12429</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/phen.12429" target="_blank" >10.1111/phen.12429</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Different fates of metabolites and small variation in chemical composition characterise frass chemistry in a specialist caterpillar

Popis výsledku v původním jazyce

Specialist and generalist insect herbivores evolved different strategies to process host-plant metabolites. We explored frass composition in Laothoe populi (L.) caterpillars (Lepidoptera: Sphingidae), a specialist moth species that is closely associated with plants of the Salicaceae (Mirb.) family. We fed the caterpillars with leaves from three willow species (Salix caprea L., S. purpurea L. and S. viminalis L.), one willow hybrid (S. x rubens Schrank) and one poplar species (Populus tremula L.). Using untargeted metabolomics, we compared the chemical composition and variation among leaf and frass samples. We quantified the chemical variation using either a simple index based on the presence and concentration of metabolites (i.e., Bray-Curtis) or an index that additionally accounts for chemical structural-compositional similarity (CSCS) among metabolites. Due to the high degree of dietary specialisation, we expected low host-specific variation in frass composition among the caterpillars. Based on literature, we further hypothesised that ingested salicinoids will be largely modified in the caterpillar gut while flavonoids will be often passively excreted. Finally, we compared chemical variation among the samples when quantified with Bray-Curtis or CSCS metrics. As expected, we found relatively low host-specific variation in the chemical composition of caterpillar frass. Our results further suggest that flavonoids were largely passively excreted by the caterpillars, while salicinoids were metabolised in the gut. Finally, we found that chemical composition measures based on Bray-Curtis overestimated the differences in chemical composition between frass and leaves, suggesting that for these type of samples CSCS measures are better suited to reflect metabolic changes more realistically.

Název v anglickém jazyce

Different fates of metabolites and small variation in chemical composition characterise frass chemistry in a specialist caterpillar

Popis výsledku anglicky

Specialist and generalist insect herbivores evolved different strategies to process host-plant metabolites. We explored frass composition in Laothoe populi (L.) caterpillars (Lepidoptera: Sphingidae), a specialist moth species that is closely associated with plants of the Salicaceae (Mirb.) family. We fed the caterpillars with leaves from three willow species (Salix caprea L., S. purpurea L. and S. viminalis L.), one willow hybrid (S. x rubens Schrank) and one poplar species (Populus tremula L.). Using untargeted metabolomics, we compared the chemical composition and variation among leaf and frass samples. We quantified the chemical variation using either a simple index based on the presence and concentration of metabolites (i.e., Bray-Curtis) or an index that additionally accounts for chemical structural-compositional similarity (CSCS) among metabolites. Due to the high degree of dietary specialisation, we expected low host-specific variation in frass composition among the caterpillars. Based on literature, we further hypothesised that ingested salicinoids will be largely modified in the caterpillar gut while flavonoids will be often passively excreted. Finally, we compared chemical variation among the samples when quantified with Bray-Curtis or CSCS metrics. As expected, we found relatively low host-specific variation in the chemical composition of caterpillar frass. Our results further suggest that flavonoids were largely passively excreted by the caterpillars, while salicinoids were metabolised in the gut. Finally, we found that chemical composition measures based on Bray-Curtis overestimated the differences in chemical composition between frass and leaves, suggesting that for these type of samples CSCS measures are better suited to reflect metabolic changes more realistically.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10616 - Entomology

Projekt

<a href="/cs/project/GF23-06855L" target="_blank" >GF23-06855L: Hybridizace jako cesta k úspěchu? Adaptivní hybridizace a její vliv na ochranu vrb před biotickými a abiotickými vlivy prostředí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Entomology

ISSN

0307-6962

e-ISSN

1365-3032

Svazek periodika

49

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

110-117

Kód UT WoS článku

001151615300001

EID výsledku v databázi Scopus

2-s2.0-85183938503