Differential gene expression and microRNA profile in <i>corpora allata-corpora cardiaca</i> of <i>Aedes aegypti</i> mosquitoes with weak juvenile hormone signalling

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00582766" target="_blank" >RIV/60077344:_____/24:00582766 - isvavai.cz</a>

Výsledek na webu

<a href="https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10007-9" target="_blank" >https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10007-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-024-10007-9" target="_blank" >10.1186/s12864-024-10007-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential gene expression and microRNA profile in <i>corpora allata-corpora cardiaca</i> of <i>Aedes aegypti</i> mosquitoes with weak juvenile hormone signalling

Popis výsledku v původním jazyce

The corpora allata-corpora cardiaca (CA-CC) is an endocrine gland complex that regulates mosquito development and reproduction through the synthesis of juvenile hormone (JH). Epoxidase (Epox) is a key enzyme in the production of JH. We recently utilized CRISPR/Cas9 to establish an epoxidase-deficient (epox(-/-)) Aedes aegypti line. The CA from epox(-/-) mutants do not synthesize epoxidated JH III but methyl farneosate (MF), a weak agonist of the JH receptor, and therefore have reduced JH signalling. Illumina sequencing was used to examine the differences in gene expression between the CA-CC from wild type (WT) and epox(-/-) adult female mosquitoes. From 18,034 identified genes, 317 were significantly differentially expressed. These genes are involved in many biological processes, including the regulation of cell proliferation and apoptosis, energy metabolism, and nutritional uptake. In addition, the same CA-CC samples were also used to examine the microRNA (miRNA) profiles of epox(-/- )and WT mosquitoes. A total of 197 miRNAs were detected, 24 of which were differentially regulated in epox(-/-) mutants. miRNA binding sites for these particular miRNAs were identified using an in silico approach, they target a total of 101 differentially expressed genes. Our results suggest that a lack of epoxidase, besides affecting JH synthesis, results in the diminishing of JH signalling that have significant effects on Ae. aegypti CA-CC transcriptome profiles, as well as its miRNA repertoire.

Název v anglickém jazyce

Differential gene expression and microRNA profile in <i>corpora allata-corpora cardiaca</i> of <i>Aedes aegypti</i> mosquitoes with weak juvenile hormone signalling

Popis výsledku anglicky

The corpora allata-corpora cardiaca (CA-CC) is an endocrine gland complex that regulates mosquito development and reproduction through the synthesis of juvenile hormone (JH). Epoxidase (Epox) is a key enzyme in the production of JH. We recently utilized CRISPR/Cas9 to establish an epoxidase-deficient (epox(-/-)) Aedes aegypti line. The CA from epox(-/-) mutants do not synthesize epoxidated JH III but methyl farneosate (MF), a weak agonist of the JH receptor, and therefore have reduced JH signalling. Illumina sequencing was used to examine the differences in gene expression between the CA-CC from wild type (WT) and epox(-/-) adult female mosquitoes. From 18,034 identified genes, 317 were significantly differentially expressed. These genes are involved in many biological processes, including the regulation of cell proliferation and apoptosis, energy metabolism, and nutritional uptake. In addition, the same CA-CC samples were also used to examine the microRNA (miRNA) profiles of epox(-/- )and WT mosquitoes. A total of 197 miRNAs were detected, 24 of which were differentially regulated in epox(-/-) mutants. miRNA binding sites for these particular miRNAs were identified using an in silico approach, they target a total of 101 differentially expressed genes. Our results suggest that a lack of epoxidase, besides affecting JH synthesis, results in the diminishing of JH signalling that have significant effects on Ae. aegypti CA-CC transcriptome profiles, as well as its miRNA repertoire.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA22-21244S" target="_blank" >GA22-21244S: Výzkum regulace vývoje a reprodukce řízené juvenilním hormonem (JH) u komárů, s využitím mutantů, kteří neprodukují JH</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BMC Genomics

ISSN

1471-2164

e-ISSN

1471-2164

Svazek periodika

25

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

113

Kód UT WoS článku

001151282500001

EID výsledku v databázi Scopus

2-s2.0-85183046576