Development, growth, and function of biofilm communities on reverse osmosis membranes desalinating tertiary effluent

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00586408" target="_blank" >RIV/60077344:_____/24:00586408 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0011916424003692?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0011916424003692?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.desal.2024.117658" target="_blank" >10.1016/j.desal.2024.117658</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development, growth, and function of biofilm communities on reverse osmosis membranes desalinating tertiary effluent

Popis výsledku v původním jazyce

Efficient tertiary effluent desalination is hindered by membrane biofouling, leading to plant downtime, shortened membrane lifespan, heightened energy consumption, and reduced permeability. While the composition of these biofouling layers is studied, little is known about bacterial succession and function. To address this, we used a benchtop reverse osmosis (RO) system to process synthetic tertiary effluent with diluted sludge. System flux monitoring tracked biofouling layer development, and RO biofilm samples were collected at early and mature stages (48, 72, 120 h). Scanning electron microscopy visualized the samples, and their 16S rRNA genes were sequenced. DNA-stable isotope probing with labeled glucose identified growing taxa in early and late biofouling stages (48, 120 h). Over time, biofouling layer biomass increased, with shifts in bacterial diversity and composition. Proteobacteria, notably oligotrophic genera, dominated early stages along with Bacteriodota, while Actinobacteria increased in mature biofilms. Functional changes included a shift from biosynthesis of cellular components like DNA, peptidoglycan, membrane lipids, and antimicrobials, to production of extracellular polysaccharides and reactive oxygen scavenging agents. In conclusion, our research enhances the understanding of biofouling dynamics within tertiary effluent desalination processes, providing insights that could improve biofouling management strategies in RO desalination systems, potentially applicable to larger-scale operations.

Název v anglickém jazyce

Development, growth, and function of biofilm communities on reverse osmosis membranes desalinating tertiary effluent

Popis výsledku anglicky

Efficient tertiary effluent desalination is hindered by membrane biofouling, leading to plant downtime, shortened membrane lifespan, heightened energy consumption, and reduced permeability. While the composition of these biofouling layers is studied, little is known about bacterial succession and function. To address this, we used a benchtop reverse osmosis (RO) system to process synthetic tertiary effluent with diluted sludge. System flux monitoring tracked biofouling layer development, and RO biofilm samples were collected at early and mature stages (48, 72, 120 h). Scanning electron microscopy visualized the samples, and their 16S rRNA genes were sequenced. DNA-stable isotope probing with labeled glucose identified growing taxa in early and late biofouling stages (48, 120 h). Over time, biofouling layer biomass increased, with shifts in bacterial diversity and composition. Proteobacteria, notably oligotrophic genera, dominated early stages along with Bacteriodota, while Actinobacteria increased in mature biofilms. Functional changes included a shift from biosynthesis of cellular components like DNA, peptidoglycan, membrane lipids, and antimicrobials, to production of extracellular polysaccharides and reactive oxygen scavenging agents. In conclusion, our research enhances the understanding of biofouling dynamics within tertiary effluent desalination processes, providing insights that could improve biofouling management strategies in RO desalination systems, potentially applicable to larger-scale operations.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Desalination

ISSN

0011-9164

e-ISSN

1873-4464

Svazek periodika

582

Číslo periodika v rámci svazku

August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

117658

Kód UT WoS článku

001220984500001

EID výsledku v databázi Scopus

2-s2.0-85190841624