A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00598342" target="_blank" >RIV/60077344:_____/24:00598342 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/24:00598265 RIV/60076658:12310/24:43908293

Výsledek na webu

<a href="https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19397" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.19397</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.19397" target="_blank" >10.1111/nph.19397</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria

Popis výsledku v původním jazyce

Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of photosystem I (PSI) in cyanobacteria, however, how the BtpA stabilized PSI remains unexplained.To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (Delta btpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only c. 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generated suppressor mutations that restore the photoautotrophy.Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the Delta btpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR.Our results show that the BtpA protein is involved in the biogenesis of photosystems at the level of regulation of tetrapyrrole biosynthesis.

Název v anglickém jazyce

A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria

Popis výsledku anglicky

Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of photosystem I (PSI) in cyanobacteria, however, how the BtpA stabilized PSI remains unexplained.To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (Delta btpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only c. 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generated suppressor mutations that restore the photoautotrophy.Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the Delta btpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR.Our results show that the BtpA protein is involved in the biogenesis of photosystems at the level of regulation of tetrapyrrole biosynthesis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

1469-8137

Svazek periodika

241

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1236-1249

Kód UT WoS článku

001107509000001

EID výsledku v databázi Scopus

2-s2.0-85177479911