Fatty acids and their derivatives from Chlorella vulgaris extracts exhibit in vitro antimicrobial activity against the honey bee pathogen Paenibacillus larvae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00599744" target="_blank" >RIV/60077344:_____/24:00599744 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/24:00599744 RIV/61989592:15310/24:73616791

Výsledek na webu

<a href="https://www.tandfonline.com/doi/full/10.1080/00218839.2021.1994264?scroll=top&needAccess=true" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/00218839.2021.1994264?scroll=top&needAccess=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00218839.2021.1994264" target="_blank" >10.1080/00218839.2021.1994264</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fatty acids and their derivatives from Chlorella vulgaris extracts exhibit in vitro antimicrobial activity against the honey bee pathogen Paenibacillus larvae

Popis výsledku v původním jazyce

The green microalga Chlorella vulgaris Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, Chlorella vulgaris has potential as a pollen supplement. We studied whether Chlorella extracts display antimicrobial properties against Paenibacillus larvae, the causative agent of the honey bee bacterial disease American foulbrood. We identified components responsible for antimicrobial activity and evaluated the added values of Chlorella as a food supplement for honey bees. Different extracts (water, acetone, methanol) were prepared from Chlorella biomass (phototrophically and heterotrophically cultivated) and screened for antimicrobial activity against ERIC I and ERIC II genotypes of P. larvae. Active acetone extracts of phototrophically cultivated Chlorella vulgaris biomass were fractioned via preparative reverse-phase chromatography. Antimicrobial activity was detected for 9 of the resulting 33 fractions. Further analysis revealed the chemical composition of the active fractions. C. vulgaris extracts showed a significant antimicrobial effect against vegetative cells and spores of P. larvae strains of ERIC I and ERIC II genotypes. The lowest MIC of the most active acetone extract was 6.3 mu g/mL for both tested genotypes. In the majority of the active fractions, monolinolenin, fatty acid linoleic acid, and methyl esters of linoleic and/or palmitic acid were identified via high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis. Based on our results, we concluded that algal C. vulgaris food supplements not only contain nutritional but also potential prophylactic properties for honey bee health.

Název v anglickém jazyce

Fatty acids and their derivatives from Chlorella vulgaris extracts exhibit in vitro antimicrobial activity against the honey bee pathogen Paenibacillus larvae

Popis výsledku anglicky

The green microalga Chlorella vulgaris Beijerinck (Chlorellaceae) is widely used as a food supplement for humans and animals. In beekeeping practice, Chlorella vulgaris has potential as a pollen supplement. We studied whether Chlorella extracts display antimicrobial properties against Paenibacillus larvae, the causative agent of the honey bee bacterial disease American foulbrood. We identified components responsible for antimicrobial activity and evaluated the added values of Chlorella as a food supplement for honey bees. Different extracts (water, acetone, methanol) were prepared from Chlorella biomass (phototrophically and heterotrophically cultivated) and screened for antimicrobial activity against ERIC I and ERIC II genotypes of P. larvae. Active acetone extracts of phototrophically cultivated Chlorella vulgaris biomass were fractioned via preparative reverse-phase chromatography. Antimicrobial activity was detected for 9 of the resulting 33 fractions. Further analysis revealed the chemical composition of the active fractions. C. vulgaris extracts showed a significant antimicrobial effect against vegetative cells and spores of P. larvae strains of ERIC I and ERIC II genotypes. The lowest MIC of the most active acetone extract was 6.3 mu g/mL for both tested genotypes. In the majority of the active fractions, monolinolenin, fatty acid linoleic acid, and methyl esters of linoleic and/or palmitic acid were identified via high-performance liquid chromatography coupled with high-resolution mass spectrometry analysis. Based on our results, we concluded that algal C. vulgaris food supplements not only contain nutritional but also potential prophylactic properties for honey bee health.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10616 - Entomology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Apicultural Research

ISSN

0021-8839

e-ISSN

2078-6913

Svazek periodika

63

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

310-322

Kód UT WoS článku

000716298500001

EID výsledku v databázi Scopus

2-s2.0-85118626218