A novel nabelschnur protein regulates segregation of the kinetoplast DNA in Trypanosoma brucei

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00603905" target="_blank" >RIV/60077344:_____/24:00603905 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17310/24:A25039N3 RIV/60076658:12310/24:43909128

Výsledek na webu

<a href="https://doi.org/10.1016/j.cub.2024.08.044" target="_blank" >https://doi.org/10.1016/j.cub.2024.08.044</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2024.08.044" target="_blank" >10.1016/j.cub.2024.08.044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel nabelschnur protein regulates segregation of the kinetoplast DNA in Trypanosoma brucei

Popis výsledku v původním jazyce

The acquisition of mitochondria was imperative for initiating eukaryogenesis and thus is a characteristic feature of eukaryotic cells.1,2 The parasitic protist Trypanosoma brucei contains a singular mitochondrion with a unique mitochondrial genome, termed the kinetoplast DNA (kDNA).3 Replication of the kDNA occurs during the G1 phase of the cell cycle, prior to the start of nuclear DNA replication.4 Although numerous proteins have been functionally characterized and identified as vital components of kDNA replication and division, the molecular mechanisms governing this highly precise process remain largely unknown.5,6 One division-related and morphologically characteristic structure that remains most enigmatic is the nabelschnur, an undefined, filament-resembling structure observed by electron microscopy between segregating daughter kDNA networks.7-9 To date, only one protein, TbLAP1, an M17 family leucyl aminopeptidase metalloprotease, is known to localize to the nabelschnur.9 While screening proteins from the T. brucei MitoTag project,10 we identified a previously uncharacterized protein with an mNeonGreen signal localizing to the kDNA as well as forming a point of connection between dividing kDNAs. Here, we demonstrate that this kDNA-associated protein, named TbNAB70, indeed localizes to the nabelschnur and plays an essential role in the segregation of newly replicated kDNAs and subsequent cytokinesis in T. brucei.

Název v anglickém jazyce

A novel nabelschnur protein regulates segregation of the kinetoplast DNA in Trypanosoma brucei

Popis výsledku anglicky

The acquisition of mitochondria was imperative for initiating eukaryogenesis and thus is a characteristic feature of eukaryotic cells.1,2 The parasitic protist Trypanosoma brucei contains a singular mitochondrion with a unique mitochondrial genome, termed the kinetoplast DNA (kDNA).3 Replication of the kDNA occurs during the G1 phase of the cell cycle, prior to the start of nuclear DNA replication.4 Although numerous proteins have been functionally characterized and identified as vital components of kDNA replication and division, the molecular mechanisms governing this highly precise process remain largely unknown.5,6 One division-related and morphologically characteristic structure that remains most enigmatic is the nabelschnur, an undefined, filament-resembling structure observed by electron microscopy between segregating daughter kDNA networks.7-9 To date, only one protein, TbLAP1, an M17 family leucyl aminopeptidase metalloprotease, is known to localize to the nabelschnur.9 While screening proteins from the T. brucei MitoTag project,10 we identified a previously uncharacterized protein with an mNeonGreen signal localizing to the kDNA as well as forming a point of connection between dividing kDNAs. Here, we demonstrate that this kDNA-associated protein, named TbNAB70, indeed localizes to the nabelschnur and plays an essential role in the segregation of newly replicated kDNAs and subsequent cytokinesis in T. brucei.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Biology

ISSN

0960-9822

e-ISSN

1879-0445

Svazek periodika

34

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

4803-4812

Kód UT WoS článku

001388001900001

EID výsledku v databázi Scopus

2-s2.0-85207472404