Sweet and sour cherry trees growing at new cultivar testing orchard and certified stock collection in Hungary are highly infected with CVA and PrVF

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00604449" target="_blank" >RIV/60077344:_____/24:00604449 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0304423824009737?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0304423824009737?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scienta.2024.113820" target="_blank" >10.1016/j.scienta.2024.113820</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sweet and sour cherry trees growing at new cultivar testing orchard and certified stock collection in Hungary are highly infected with CVA and PrVF

Popis výsledku v původním jazyce

Hungary has a long tradition of sour cherry breeding. Clone selection for new cultivars with desirable traits recently replaced by classical breeding, and new cultivar candidates waiting for evaluations of their phenotypes are maintained at a testing orchard. The viral status of these new sour cherry cultivar candidates was tested using small-RNA high-throughput sequencing (HTS) as an unbiased diagnostic method. Moreover, the viromes of certified stock collections of sour cherry and sweet cherry growing in the vicinity of the testing orchard were also determined. Bioinformatic analysis of the sRNA HTS was validated using unbiased methods, revealing that the trees had very high rates of cherry virus A (CVA) and Prunus virus F (PrVF) infections. The latter virus was described in Hungary for the first time. While the stock collection was free from Prunus necrotic ringspot virus (PNRSV), this regulated virus was present at the testing orchard. As a follow-up study, the survey of the testing orchard was repeated after two years using RT-PCR with a detailed survey of the stock collection. The infection pattern of the viruses suggests the spread of PNRSV via pollen at the testing orchard, where the trees can bloom. Notably, the strains of both CVA and PrVF varied to a large extent. A low concentration of virus-derived small RNAs suggests that they do not induce strong antiviral RNAi, which could explain why their infections can be latent, without visible symptoms. However, to determine the impacts of frequent coinfections on fruit yield and tree health, which could be further altered by the changing climate, further investigations will be needed in the future.

Název v anglickém jazyce

Sweet and sour cherry trees growing at new cultivar testing orchard and certified stock collection in Hungary are highly infected with CVA and PrVF

Popis výsledku anglicky

Hungary has a long tradition of sour cherry breeding. Clone selection for new cultivars with desirable traits recently replaced by classical breeding, and new cultivar candidates waiting for evaluations of their phenotypes are maintained at a testing orchard. The viral status of these new sour cherry cultivar candidates was tested using small-RNA high-throughput sequencing (HTS) as an unbiased diagnostic method. Moreover, the viromes of certified stock collections of sour cherry and sweet cherry growing in the vicinity of the testing orchard were also determined. Bioinformatic analysis of the sRNA HTS was validated using unbiased methods, revealing that the trees had very high rates of cherry virus A (CVA) and Prunus virus F (PrVF) infections. The latter virus was described in Hungary for the first time. While the stock collection was free from Prunus necrotic ringspot virus (PNRSV), this regulated virus was present at the testing orchard. As a follow-up study, the survey of the testing orchard was repeated after two years using RT-PCR with a detailed survey of the stock collection. The infection pattern of the viruses suggests the spread of PNRSV via pollen at the testing orchard, where the trees can bloom. Notably, the strains of both CVA and PrVF varied to a large extent. A low concentration of virus-derived small RNAs suggests that they do not induce strong antiviral RNAi, which could explain why their infections can be latent, without visible symptoms. However, to determine the impacts of frequent coinfections on fruit yield and tree health, which could be further altered by the changing climate, further investigations will be needed in the future.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientia horticulturae

ISSN

0304-4238

e-ISSN

1879-1018

Svazek periodika

338

Číslo periodika v rámci svazku

Dec 1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

14

Strana od-do

113820

Kód UT WoS článku

001363604000001

EID výsledku v databázi Scopus

2-s2.0-85209547008