Insights into extracellular vesicle biogenesis and secretion of the tapeworm Hymenolepis diminuta: host interaction and cultivation dynamics q

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00617104" target="_blank" >RIV/60077344:_____/25:00617104 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43909995

Výsledek na webu

<a href="https://doi.org/10.1016/j.ijpara.2024.11.005" target="_blank" >https://doi.org/10.1016/j.ijpara.2024.11.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijpara.2024.11.005" target="_blank" >10.1016/j.ijpara.2024.11.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insights into extracellular vesicle biogenesis and secretion of the tapeworm Hymenolepis diminuta: host interaction and cultivation dynamics q

Popis výsledku v původním jazyce

Extracellular vesicles (EVs) from parasites have been identified as potent modulators of host-parasite interactions. However, their biogenesis and secretory activity are still poorly understood. Here we present a comprehensive examination of the secretory dynamics of two distinct EV fractions isolated from the adult tapeworm Hymenolepis diminuta. Additionally, we perform a detailed analysis of changes in proteomic content and morphology during EV secretion, utilising electron tomography to shed light on a previously described novel mechanism of EV biogenesis via bead-like protrusion. Our findings reveal a significant decrease in EV secretion between 24 and 48 h of in vitro cultivation when external host stimuli are no longer present. Finally, this study addresses, for the first known time, the potential bias in EV analysis resulting from extended in vitro cultivation of model parasites. (c) 2024 Published by Elsevier Ltd on behalf of Australian Society for Parasitology.

Název v anglickém jazyce

Insights into extracellular vesicle biogenesis and secretion of the tapeworm Hymenolepis diminuta: host interaction and cultivation dynamics q

Popis výsledku anglicky

Extracellular vesicles (EVs) from parasites have been identified as potent modulators of host-parasite interactions. However, their biogenesis and secretory activity are still poorly understood. Here we present a comprehensive examination of the secretory dynamics of two distinct EV fractions isolated from the adult tapeworm Hymenolepis diminuta. Additionally, we perform a detailed analysis of changes in proteomic content and morphology during EV secretion, utilising electron tomography to shed light on a previously described novel mechanism of EV biogenesis via bead-like protrusion. Our findings reveal a significant decrease in EV secretion between 24 and 48 h of in vitro cultivation when external host stimuli are no longer present. Finally, this study addresses, for the first known time, the potential bias in EV analysis resulting from extended in vitro cultivation of model parasites. (c) 2024 Published by Elsevier Ltd on behalf of Australian Society for Parasitology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal for Parasitology

ISSN

0020-7519

e-ISSN

1879-0135

Svazek periodika

55

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

69-77

Kód UT WoS článku

001417250200001

EID výsledku v databázi Scopus

2-s2.0-85212321228