Multiple plastid losses within photosynthetic stramenopiles revealed by comprehensive phylogenomics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00617429" target="_blank" >RIV/60077344:_____/25:00617429 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17310/25:A26039VB RIV/00216208:11310/25:10496529

Výsledek na webu

<a href="https://doi.org/10.1016/j.cub.2024.11.065" target="_blank" >https://doi.org/10.1016/j.cub.2024.11.065</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2024.11.065" target="_blank" >10.1016/j.cub.2024.11.065</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple plastid losses within photosynthetic stramenopiles revealed by comprehensive phylogenomics

Popis výsledku v původním jazyce

Ochrophyta is avast and morphologically diverse group of algae with complex plastids, including familiar taxa with fundamental ecological importance (diatoms or kelp) and a wealth of lesser-known and obscure organisms. The sheer diversity of ochrophytes poses a challenge for reconstructing their phylogeny, with major gaps in sampling and an unsettled placement of particular taxa yet to be tackled. We sequenced transcriptomes from 25 strategically selected representatives and used these data to build the most taxonomically comprehensive ochrophyte-centered phylogenomic supermatrix to date. We employed a combination of approaches to reconstruct and critically evaluate the relationships among ochrophytes. While generally congruent with previous analyses, the updated ochrophyte phylogenomic tree resolved the position of several taxa with previously uncertain placement and supported a redefinition of the classes Picophagea and Synchromophyceae. Our results indicated that the heterotrophic, plastid-lacking heliozoan Actinophrys sol is not a sister lineage of ochrophytes, as proposed recently, but rather phylogenetically nested among them, implying that it lacks a plastid due to loss. In addition, we found the heterotrophic ochrophyte Picophagus flagellatus to lack all hallmark plastid genes yet to exhibit mitochondrial proteins that seem to be genetic footprints of a lost plastid organelle. We thus document, for the first time, plastid loss in two separate ochrophyte lineages. Furthermore, by exploring eDNA data, we enrich the ochrophyte phylogenetic tree by identifying five novel uncultured class-level lineages. Altogether, our study provides a new framework for reconstructing trait evolution in ochrophytes and demonstrates that plastid loss is more common than previously thought.

Název v anglickém jazyce

Multiple plastid losses within photosynthetic stramenopiles revealed by comprehensive phylogenomics

Popis výsledku anglicky

Ochrophyta is avast and morphologically diverse group of algae with complex plastids, including familiar taxa with fundamental ecological importance (diatoms or kelp) and a wealth of lesser-known and obscure organisms. The sheer diversity of ochrophytes poses a challenge for reconstructing their phylogeny, with major gaps in sampling and an unsettled placement of particular taxa yet to be tackled. We sequenced transcriptomes from 25 strategically selected representatives and used these data to build the most taxonomically comprehensive ochrophyte-centered phylogenomic supermatrix to date. We employed a combination of approaches to reconstruct and critically evaluate the relationships among ochrophytes. While generally congruent with previous analyses, the updated ochrophyte phylogenomic tree resolved the position of several taxa with previously uncertain placement and supported a redefinition of the classes Picophagea and Synchromophyceae. Our results indicated that the heterotrophic, plastid-lacking heliozoan Actinophrys sol is not a sister lineage of ochrophytes, as proposed recently, but rather phylogenetically nested among them, implying that it lacks a plastid due to loss. In addition, we found the heterotrophic ochrophyte Picophagus flagellatus to lack all hallmark plastid genes yet to exhibit mitochondrial proteins that seem to be genetic footprints of a lost plastid organelle. We thus document, for the first time, plastid loss in two separate ochrophyte lineages. Furthermore, by exploring eDNA data, we enrich the ochrophyte phylogenetic tree by identifying five novel uncultured class-level lineages. Altogether, our study provides a new framework for reconstructing trait evolution in ochrophytes and demonstrates that plastid loss is more common than previously thought.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Biology

ISSN

0960-9822

e-ISSN

1879-0445

Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

26

Strana od-do

"483"-"499.e8"

Kód UT WoS článku

001422585400001

EID výsledku v databázi Scopus

2-s2.0-85216475810