Metabolomic signatures associated with cold adaptation and seasonal acclimation of Drosophila: profiling of 43 species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00618044" target="_blank" >RIV/60077344:_____/25:00618044 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43909927

Výsledek na webu

<a href="https://journals.biologists.com/jeb/article/228/5/JEB250076/367323/Metabolomic-signatures-associated-with-cold" target="_blank" >https://journals.biologists.com/jeb/article/228/5/JEB250076/367323/Metabolomic-signatures-associated-with-cold</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/jeb.250076" target="_blank" >10.1242/jeb.250076</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metabolomic signatures associated with cold adaptation and seasonal acclimation of Drosophila: profiling of 43 species

Popis výsledku v původním jazyce

Cold tolerance is a key determinant of poleward colonization in insects. However, the physiological basis underlying interspecific differences in cold tolerance is not fully understood. Here, we analyzed cold tolerance and metabolomic profiles in warm- and coldacclimated phenotypes of 43 Drosophila species representing a latitudinal gradient from the tropics to the boreal zone. We found a strong positive correlation between cold tolerance and climatic variables associated with habitat seasonality and temperature. Including the effects of cold acclimation, we found most species have similar ‘safety margins’, measured as the difference between the average environmental temperature and the lower lethal temperature. Searching for metabolomic signatures of cold tolerance, we found that the warm-acclimated flies of cold-hardy species had moderately but significantly higher constitutive signals of putative cryoprotectants such as trehalose, glucose, glycerol and mannitol/sorbitol. Cold acclimation (and the transition to a winter dormant phenotype) resulted in a strong accumulation of myoinositol, which occurred only in species of the virilis group. Other temperate and boreal species either showed only moderate, idiosyncratic accumulations of sugars/polyols and free amino acids, or did not accumulate any ‘classical’ cryoprotectant at all. Thus, our results suggest that the colonization of boreal regions by Drosophila does not necessarily depend on the seasonal accumulation of classical cryoprotectants. In contrast, virtually all cold-acclimated species showed a significant increase in products of phospholipid catabolism, suggesting that remodeling of biological membranes is a clear and ubiquitous signature of cold acclimation in Drosophila.

Název v anglickém jazyce

Metabolomic signatures associated with cold adaptation and seasonal acclimation of Drosophila: profiling of 43 species

Popis výsledku anglicky

Cold tolerance is a key determinant of poleward colonization in insects. However, the physiological basis underlying interspecific differences in cold tolerance is not fully understood. Here, we analyzed cold tolerance and metabolomic profiles in warm- and coldacclimated phenotypes of 43 Drosophila species representing a latitudinal gradient from the tropics to the boreal zone. We found a strong positive correlation between cold tolerance and climatic variables associated with habitat seasonality and temperature. Including the effects of cold acclimation, we found most species have similar ‘safety margins’, measured as the difference between the average environmental temperature and the lower lethal temperature. Searching for metabolomic signatures of cold tolerance, we found that the warm-acclimated flies of cold-hardy species had moderately but significantly higher constitutive signals of putative cryoprotectants such as trehalose, glucose, glycerol and mannitol/sorbitol. Cold acclimation (and the transition to a winter dormant phenotype) resulted in a strong accumulation of myoinositol, which occurred only in species of the virilis group. Other temperate and boreal species either showed only moderate, idiosyncratic accumulations of sugars/polyols and free amino acids, or did not accumulate any ‘classical’ cryoprotectant at all. Thus, our results suggest that the colonization of boreal regions by Drosophila does not necessarily depend on the seasonal accumulation of classical cryoprotectants. In contrast, virtually all cold-acclimated species showed a significant increase in products of phospholipid catabolism, suggesting that remodeling of biological membranes is a clear and ubiquitous signature of cold acclimation in Drosophila.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/GA23-06518S" target="_blank" >GA23-06518S: Diversita a fyziologický význam malých kryoprotektivních molekul octomilek: zaměřeno na mitochondriální membrány.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Biology

ISSN

0022-0949

e-ISSN

1477-9145

Svazek periodika

228

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

250076

Kód UT WoS článku

001461192200004

EID výsledku v databázi Scopus

2-s2.0-105000256507