Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00619033" target="_blank" >RIV/60077344:_____/25:00619033 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/25:00619033 RIV/60076658:12310/25:43909896 RIV/61988987:17310/25:A2603CM2 RIV/00216208:11310/25:10503687

Výsledek na webu

<a href="https://www.nature.com/articles/s41594-024-01450-z" target="_blank" >https://www.nature.com/articles/s41594-024-01450-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41594-024-01450-z" target="_blank" >10.1038/s41594-024-01450-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough

Popis výsledku v původním jazyce

Transfer RNAs (tRNAs) serve as a dictionary for the ribosome translating the genetic message from mRNA into a polypeptide chain. In addition to this canonical role, tRNAs are involved in other processes such as programmed stop codon readthrough (SC-RT). There, tRNAs with near-cognate anticodons to stop codons must outcompete release factors and incorporate into the ribosomal decoding center to prevent termination and allow translation to continue. However, not all near-cognate tRNAs promote efficient SC-RT. Here, with the help of Saccharomyces cerevisiae and Trypanosomabrucei, we demonstrate that those tRNAs that promote efficient SC-RT establish critical contacts between their anticodon stem (AS) and ribosomal proteins Rps30/eS30 and Rps25/eS25 forming the decoding site. Unexpectedly, the length and well-defined nature of the AS determine the strength of these contacts, which is reflected in organisms with reassigned stop codons. These findings open an unexplored direction in tRNA biology that should facilitate the design of artificial tRNAs with specifically altered decoding abilities.

Název v anglickém jazyce

Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough

Popis výsledku anglicky

Transfer RNAs (tRNAs) serve as a dictionary for the ribosome translating the genetic message from mRNA into a polypeptide chain. In addition to this canonical role, tRNAs are involved in other processes such as programmed stop codon readthrough (SC-RT). There, tRNAs with near-cognate anticodons to stop codons must outcompete release factors and incorporate into the ribosomal decoding center to prevent termination and allow translation to continue. However, not all near-cognate tRNAs promote efficient SC-RT. Here, with the help of Saccharomyces cerevisiae and Trypanosomabrucei, we demonstrate that those tRNAs that promote efficient SC-RT establish critical contacts between their anticodon stem (AS) and ribosomal proteins Rps30/eS30 and Rps25/eS25 forming the decoding site. Unexpectedly, the length and well-defined nature of the AS determine the strength of these contacts, which is reflected in organisms with reassigned stop codons. These findings open an unexplored direction in tRNA biology that should facilitate the design of artificial tRNAs with specifically altered decoding abilities.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Structural & Molecular Biology

ISSN

1545-9993

e-ISSN

1545-9985

Svazek periodika

32

Číslo periodika v rámci svazku

January 13

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

662-674

Kód UT WoS článku

001395439100001

EID výsledku v databázi Scopus

2-s2.0-85217547932