Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00619121" target="_blank" >RIV/60077344:_____/25:00619121 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17310/25:A2603CLY RIV/60076658:12310/25:43909897 RIV/00216208:11310/25:10497809

Výsledek na webu

<a href="https://doi.org/10.1016/j.cub.2025.02.032" target="_blank" >https://doi.org/10.1016/j.cub.2025.02.032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2025.02.032" target="_blank" >10.1016/j.cub.2025.02.032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes

Popis výsledku v původním jazyce

Diplonemids are among the most abundant and species-rich protists in the oceans. Marine heterotrophic flagellates, including diplonemids, have been suggested to play important roles in global biogeochemical cles. Diplonemids are also the sister taxon of kinetoplastids, home to trypanosomatid parasites of global health importance, and thus are informative about the evolution of kinetoplastid biology. However, genomic and cellular complement that underpins diplonemids' highly successful lifestyle is underexplored. At the same time, our framework describing cellular processes may not be as broadly applicable as sumed, as it is largely derived from animal and fungal model organisms, a small subset of extant eukaryotic diversity. In addition to uniquely evolved machinery in animals and fungi, there exist components sporadic (i.e., patchy) distributions across other eukaryotes. A most intriguing subset are components (spacing diaeresis>tnarlogs) stochastically present in a wide range of eukaryotes but lost in animal and/or fungal models. Such components are considered exotic curiosities but may be relevant to inferences about the complexity of the last eukaryotic common ancestor (LECA) and frameworks of modern cell biology. Here, we comparative genomics and phylogenetics to comprehensively assess the membrane-trafficking system diplonemids. They possess several proteins thought of as kinetoplastid specific, as well as an extensive set of patchy proteins, including jo<spacing diaeresis>tnarlogs. Diplonemids apparently function with endomembrane machinery distinct from existing cell biological models but comparable with other free-living heterotrophic protists, highlighting the importance of including such exotic components when considering different models ancient eukaryotic genomic complexity and the cell biology of non-opisthokont organisms.

Název v anglickém jazyce

Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes

Popis výsledku anglicky

Diplonemids are among the most abundant and species-rich protists in the oceans. Marine heterotrophic flagellates, including diplonemids, have been suggested to play important roles in global biogeochemical cles. Diplonemids are also the sister taxon of kinetoplastids, home to trypanosomatid parasites of global health importance, and thus are informative about the evolution of kinetoplastid biology. However, genomic and cellular complement that underpins diplonemids' highly successful lifestyle is underexplored. At the same time, our framework describing cellular processes may not be as broadly applicable as sumed, as it is largely derived from animal and fungal model organisms, a small subset of extant eukaryotic diversity. In addition to uniquely evolved machinery in animals and fungi, there exist components sporadic (i.e., patchy) distributions across other eukaryotes. A most intriguing subset are components (spacing diaeresis>tnarlogs) stochastically present in a wide range of eukaryotes but lost in animal and/or fungal models. Such components are considered exotic curiosities but may be relevant to inferences about the complexity of the last eukaryotic common ancestor (LECA) and frameworks of modern cell biology. Here, we comparative genomics and phylogenetics to comprehensively assess the membrane-trafficking system diplonemids. They possess several proteins thought of as kinetoplastid specific, as well as an extensive set of patchy proteins, including jo<spacing diaeresis>tnarlogs. Diplonemids apparently function with endomembrane machinery distinct from existing cell biological models but comparable with other free-living heterotrophic protists, highlighting the importance of including such exotic components when considering different models ancient eukaryotic genomic complexity and the cell biology of non-opisthokont organisms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Biology

ISSN

0960-9822

e-ISSN

1879-0445

Svazek periodika

35

Číslo periodika v rámci svazku

APR 2025

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

7

Kód UT WoS článku

001465193300001

EID výsledku v databázi Scopus

2-s2.0-86000745219