Navigating the seven seas of arthropod collection protocols: Metabarcoding arthropod diversity in a tropical forest

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00638752" target="_blank" >RIV/60077344:_____/25:00638752 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/25:73634566

Výsledek na webu

<a href="https://besjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/2041-210X.70135" target="_blank" >https://besjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/2041-210X.70135</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/2041-210X.70135" target="_blank" >10.1111/2041-210X.70135</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Navigating the seven seas of arthropod collection protocols: Metabarcoding arthropod diversity in a tropical forest

Popis výsledku v původním jazyce

Insects and other arthropods represent one of the most diverse groups of life on Earth, with an estimated 70% of species awaiting discovery. Advances in DNA-based tools have accelerated species discovery, while high-throughput sequencing has enabled large-scale biodiversity surveys. Arthropods also exhibit immense ecological, habitat and distributional diversity, requiring multiple sampling methods to effectively capture this variation. Comparisons of results provided by these methods, once the samples are processed by DNA metabarcoding, have rarely been evaluated.nnTo address this gap, we employed seven of the most widely used arthropod bulk-sampling protocols across 25 subplots (20 × 20 m) in a tropical rainforest on Barro Colorado Island, Panama, during the wet and dry seasons. We used Berlese-Tullgren funnels, Winkler extractors and pitfall traps to target soil and leaf litter fauna, beating to sample arthropods resting on vegetation, and polytraps, Malaise and light traps to survey flying fauna. All 350 samples were processed with COI metabarcoding, and resulting sequences were assigned to known Barcode Index Numbers (BINs) using the mBRAVE platform.nnBeyond documenting the magnitude of arthropod richness in one of the world's most biodiverse ecosystems, our study aimed to identify the most cost-efficient sampling methods (USD/BIN) for capturing broad arthropod diversity in a complex environment. We detected ca. 10,000 arthropod species within a combined hectare of tropical lowland rainforest, with each sampling subplot revealing significant diversity (2227 ± 28 BINs). Our findings highlight the utility of method-specific targeting, from Berlese-Tullgren funnels efficiently detecting soil micro-arthropods to generalist approaches such as light traps, which captured a broad diversity of well-studied taxa (e.g. moths).nnOur results demonstrate that DNA metabarcoding enhances species recovery beyond the target taxa. Based on our findings, we recommend a combined approach using flight-intercept and soil-targeting traps, with Malaise traps effectively capturing flying and ground dwelling/emerging arthropods. Pitfall traps, comparatively simple and inexpensive, recover a wide range of arthropods and offer a practical starting point for baseline surveys.

Název v anglickém jazyce

Navigating the seven seas of arthropod collection protocols: Metabarcoding arthropod diversity in a tropical forest

Popis výsledku anglicky

Insects and other arthropods represent one of the most diverse groups of life on Earth, with an estimated 70% of species awaiting discovery. Advances in DNA-based tools have accelerated species discovery, while high-throughput sequencing has enabled large-scale biodiversity surveys. Arthropods also exhibit immense ecological, habitat and distributional diversity, requiring multiple sampling methods to effectively capture this variation. Comparisons of results provided by these methods, once the samples are processed by DNA metabarcoding, have rarely been evaluated.nnTo address this gap, we employed seven of the most widely used arthropod bulk-sampling protocols across 25 subplots (20 × 20 m) in a tropical rainforest on Barro Colorado Island, Panama, during the wet and dry seasons. We used Berlese-Tullgren funnels, Winkler extractors and pitfall traps to target soil and leaf litter fauna, beating to sample arthropods resting on vegetation, and polytraps, Malaise and light traps to survey flying fauna. All 350 samples were processed with COI metabarcoding, and resulting sequences were assigned to known Barcode Index Numbers (BINs) using the mBRAVE platform.nnBeyond documenting the magnitude of arthropod richness in one of the world's most biodiverse ecosystems, our study aimed to identify the most cost-efficient sampling methods (USD/BIN) for capturing broad arthropod diversity in a complex environment. We detected ca. 10,000 arthropod species within a combined hectare of tropical lowland rainforest, with each sampling subplot revealing significant diversity (2227 ± 28 BINs). Our findings highlight the utility of method-specific targeting, from Berlese-Tullgren funnels efficiently detecting soil micro-arthropods to generalist approaches such as light traps, which captured a broad diversity of well-studied taxa (e.g. moths).nnOur results demonstrate that DNA metabarcoding enhances species recovery beyond the target taxa. Based on our findings, we recommend a combined approach using flight-intercept and soil-targeting traps, with Malaise traps effectively capturing flying and ground dwelling/emerging arthropods. Pitfall traps, comparatively simple and inexpensive, recover a wide range of arthropods and offer a practical starting point for baseline surveys.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

<a href="/cs/project/GA25-17499S" target="_blank" >GA25-17499S: Je populační dynamika hmyzu v tropickém deštném lese řízena primárně dostupností zdrojů, tlakem predátorů nebo variabilitou klimatu?</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Methods in Ecology and Evolution

ISSN

2041-210X

e-ISSN

2041-2096

Svazek periodika

16

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

2395-2407

Kód UT WoS článku

001563571600001

EID výsledku v databázi Scopus

2-s2.0-105014730590