Hidden Plasmodium diversity revealed in southeastern Asian passerines using next-generation amplicon sequencing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00639443" target="_blank" >RIV/60077344:_____/25:00639443 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43909849

Výsledek na webu

<a href="https://doi.org/10.1016/j.crpvbd.2025.100319" target="_blank" >https://doi.org/10.1016/j.crpvbd.2025.100319</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.crpvbd.2025.100319" target="_blank" >10.1016/j.crpvbd.2025.100319</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hidden Plasmodium diversity revealed in southeastern Asian passerines using next-generation amplicon sequencing

Popis výsledku v původním jazyce

Monitoring haemosporidian (Genus Plasmodium) infections in passerine birds is essential for understanding the intricate dynamics of avian malaria and its implications for ecology and evolution of avian populations. In this study, we investigated the prevalence and diversity of malaria infections caused by Plasmodium species in three passerine species in Cat-Tien National Park, Vietnam. Using next-generation amplicon sequencing (NGS) of haemosporidian cytochrome b gene, we identified two known and ten novel Plasmodium lineages. Our genetic analysis revealed a high rate of Plasmodium infections in the little spiderhunter (Arachnothera longirostra, Nectariniidae), the white-rumped shama (Copsychus malabaricus, Muscicapidae) and the blue-winged pitta (Pitta moluccensis, Pittidae). Species delimitation methods identified five distinct operational taxonomic units (OTUs), consistently across the majority of the used methods. Each of the passerine species was infected with a specific subset of the total Plasmodium diversity. Phylogenetic analysis showed that sympatric Plasmodium OTUs are not closely related and possess overlapping host preferences. Our findings may reflect differences in habitat use, such as the vertical strata occupied by different bird species, which contribute to varying exposure levels to suitable vectors, thereby influencing infection rates and parasite diversity. Our findings corroborate the view that avian malaria parasites are not uniformly opportunistic, rather, their distribution is filtered by host identity and ecology. Understanding these dynamics is crucial for avian conservation and broader ecological studies, as avian malaria serves as a model for studying parasite-vector-host co-evolution and the impact of environmental changes on disease dynamics.

Název v anglickém jazyce

Hidden Plasmodium diversity revealed in southeastern Asian passerines using next-generation amplicon sequencing

Popis výsledku anglicky

Monitoring haemosporidian (Genus Plasmodium) infections in passerine birds is essential for understanding the intricate dynamics of avian malaria and its implications for ecology and evolution of avian populations. In this study, we investigated the prevalence and diversity of malaria infections caused by Plasmodium species in three passerine species in Cat-Tien National Park, Vietnam. Using next-generation amplicon sequencing (NGS) of haemosporidian cytochrome b gene, we identified two known and ten novel Plasmodium lineages. Our genetic analysis revealed a high rate of Plasmodium infections in the little spiderhunter (Arachnothera longirostra, Nectariniidae), the white-rumped shama (Copsychus malabaricus, Muscicapidae) and the blue-winged pitta (Pitta moluccensis, Pittidae). Species delimitation methods identified five distinct operational taxonomic units (OTUs), consistently across the majority of the used methods. Each of the passerine species was infected with a specific subset of the total Plasmodium diversity. Phylogenetic analysis showed that sympatric Plasmodium OTUs are not closely related and possess overlapping host preferences. Our findings may reflect differences in habitat use, such as the vertical strata occupied by different bird species, which contribute to varying exposure levels to suitable vectors, thereby influencing infection rates and parasite diversity. Our findings corroborate the view that avian malaria parasites are not uniformly opportunistic, rather, their distribution is filtered by host identity and ecology. Understanding these dynamics is crucial for avian conservation and broader ecological studies, as avian malaria serves as a model for studying parasite-vector-host co-evolution and the impact of environmental changes on disease dynamics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Research in Parasitology and Vector-Borne Diseases

ISSN

2667-114X

e-ISSN

2667-114X

Svazek periodika

8

Číslo periodika v rámci svazku

SEP 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

100319

Kód UT WoS článku

001577756900001

EID výsledku v databázi Scopus

2-s2.0-105016474246