Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00640498" target="_blank" >RIV/60077344:_____/25:00640498 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/25:43910396

Výsledek na webu

<a href="https://doi.org/10.1186/s13071-025-07068-4" target="_blank" >https://doi.org/10.1186/s13071-025-07068-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13071-025-07068-4" target="_blank" >10.1186/s13071-025-07068-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos

Popis výsledku v původním jazyce

BackgroundCryptosporidium is a globally prevalent parasite that infects the gastrointestinal tract of a large number of vertebrates. The Cryptosporidium sp. lizard genotype is found almost exclusively in geckos but has also been detected in a corn snake. In this study, the biology and genetic variability of the lizard genotype were investigated.MethodsThe genetic variability of the Cryptosporidium sp. lizard genotype was analysed by polymerase chain reaction (PCR) and Sanger sequencing of the SSU rRNA, actin, HSP70 and COWP genes. Its biological characteristics, including oocyst size, tissue tropism and associated pathology, were investigated using light microscopy, electron microscopy and histological techniques. Experimental infections were performed to assess host specificity and infectivity.ResultsPhylogenetic analysis of the target genes confirmed that the Cryptosporidium sp. lizard genotype is genetically distinct from other Cryptosporidium species. Experimental infections showed that chickens, mice and corn snakes were not susceptible to this genotype, as no infection was detected. In contrast, leopard geckos began to excrete oocysts between 6 and 8 days post-infection, although they showed no clinical symptoms. Excretion of oocysts continued for over 200 days. Oocysts isolated from naturally infected leopard and smooth knob-tailed geckos were morphometrically identical to those from experimentally infected leopard geckos and measured 5.89 x 4.79 mu m. The parasite mainly colonised the stomach, where the highest intensity of infection was observed, but was also found throughout the intestine and in the lungs.ConclusionsDue to the distinct genetic differences, host range and tissue tropism, we propose the name Cryptosporidium geckonae n. sp. for this organism, which was previously referred to as Cryptosporidium sp. lizard genotype.

Název v anglickém jazyce

Cryptosporidium geckonae n. sp. (Apicomplexa: Cryptosporidiidae) in geckos

Popis výsledku anglicky

BackgroundCryptosporidium is a globally prevalent parasite that infects the gastrointestinal tract of a large number of vertebrates. The Cryptosporidium sp. lizard genotype is found almost exclusively in geckos but has also been detected in a corn snake. In this study, the biology and genetic variability of the lizard genotype were investigated.MethodsThe genetic variability of the Cryptosporidium sp. lizard genotype was analysed by polymerase chain reaction (PCR) and Sanger sequencing of the SSU rRNA, actin, HSP70 and COWP genes. Its biological characteristics, including oocyst size, tissue tropism and associated pathology, were investigated using light microscopy, electron microscopy and histological techniques. Experimental infections were performed to assess host specificity and infectivity.ResultsPhylogenetic analysis of the target genes confirmed that the Cryptosporidium sp. lizard genotype is genetically distinct from other Cryptosporidium species. Experimental infections showed that chickens, mice and corn snakes were not susceptible to this genotype, as no infection was detected. In contrast, leopard geckos began to excrete oocysts between 6 and 8 days post-infection, although they showed no clinical symptoms. Excretion of oocysts continued for over 200 days. Oocysts isolated from naturally infected leopard and smooth knob-tailed geckos were morphometrically identical to those from experimentally infected leopard geckos and measured 5.89 x 4.79 mu m. The parasite mainly colonised the stomach, where the highest intensity of infection was observed, but was also found throughout the intestine and in the lungs.ConclusionsDue to the distinct genetic differences, host range and tissue tropism, we propose the name Cryptosporidium geckonae n. sp. for this organism, which was previously referred to as Cryptosporidium sp. lizard genotype.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasites & Vectors

ISSN

1756-3305

e-ISSN

1756-3305

Svazek periodika

18

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

420

Kód UT WoS článku

001597433600005

EID výsledku v databázi Scopus

2-s2.0-105019743992