Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00641685" target="_blank" >RIV/60077344:_____/25:00641685 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/25:43910335

Výsledek na webu

<a href="https://doi.org/10.1038/s42003-025-08883-2" target="_blank" >https://doi.org/10.1038/s42003-025-08883-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s42003-025-08883-2" target="_blank" >10.1038/s42003-025-08883-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi

Popis výsledku v původním jazyce

Kinetoplast (k) DNA, a specialized form of mitochondrial DNA in trypanosomes, is composed of catenated maxicircles and minicircles, and is essential for the survival of these parasitic protists. In this study, we provide a detailed description of the near-complete minicircle complement of Trypanosoma musculi, a globally distributed, mouse-specific haemoflagellate. We have identified 39 minicircle classes, which can be grouped via their structural organization into five distinct categories. Using RNA-seq, we have reconstructed mitochondrial transcripts of ATP6, RPS12, CYTB, COX2 and COX3, all of which undergo canonical editing that renders them translatable, as well as abnormally edited transcripts of four respiratory Complex I subunits. Additionally, through a combined analysis of the kDNA minicircles, edited mRNAs and small RNAs, we identified 108 guide (g)RNA genes and further categorized their conservation in comparison with Trypanosoma lewisi, an opportunistic human parasite. Moreover, we demonstrate a small respiratory Complex I in T. musculi, highlighting the functional consequences of missing gRNAs. Finally, we propose a model for the evolutionary maintenance of gRNAs, contributing to our understanding of kDNA dynamics in trypanosomes and other kinetoplastid flagellates.

Název v anglickém jazyce

Kinetoplast DNA structure, RNA editing patterns and small respiratory Complex I in Trypanosoma musculi

Popis výsledku anglicky

Kinetoplast (k) DNA, a specialized form of mitochondrial DNA in trypanosomes, is composed of catenated maxicircles and minicircles, and is essential for the survival of these parasitic protists. In this study, we provide a detailed description of the near-complete minicircle complement of Trypanosoma musculi, a globally distributed, mouse-specific haemoflagellate. We have identified 39 minicircle classes, which can be grouped via their structural organization into five distinct categories. Using RNA-seq, we have reconstructed mitochondrial transcripts of ATP6, RPS12, CYTB, COX2 and COX3, all of which undergo canonical editing that renders them translatable, as well as abnormally edited transcripts of four respiratory Complex I subunits. Additionally, through a combined analysis of the kDNA minicircles, edited mRNAs and small RNAs, we identified 108 guide (g)RNA genes and further categorized their conservation in comparison with Trypanosoma lewisi, an opportunistic human parasite. Moreover, we demonstrate a small respiratory Complex I in T. musculi, highlighting the functional consequences of missing gRNAs. Finally, we propose a model for the evolutionary maintenance of gRNAs, contributing to our understanding of kDNA dynamics in trypanosomes and other kinetoplastid flagellates.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Communications Biology

ISSN

2399-3642

e-ISSN

2399-3642

Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

1544

Kód UT WoS článku

001610138200003

EID výsledku v databázi Scopus

2-s2.0-105021068033