High-temporal resolution of microbial food web dynamics and structure during phytoplankton blooms in the Baltic Sea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00646942" target="_blank" >RIV/60077344:_____/25:00646942 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1111/1462-2920.70190" target="_blank" >https://doi.org/10.1111/1462-2920.70190</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1462-2920.70190" target="_blank" >10.1111/1462-2920.70190</a>

Jazyk výsledku

angličtina

Název v původním jazyce

High-temporal resolution of microbial food web dynamics and structure during phytoplankton blooms in the Baltic Sea

Popis výsledku v původním jazyce

Heterotrophic nanoflagellates (HNF) are a key component of the microbial food webs, playing an essential role in nutrient recycling and energy transfer in aquatic ecosystems. They have been typically considered to be bacterivores, but they can also be omnivorous (feeding on prokaryotes and other eukaryotes) and predatory grazers (feeding on other eukaryotes). Here, we combine CARD-FISH with both short and long-amplicon sequencing to resolve the dynamics of key HNF groups during two high-frequency sampling campaigns in spring (March-May) and autumn (September-November) phytoplankton blooms in the coastal waters of the Baltic Sea. This approach allowed us to resolve the microbial food web dynamics within HNF communities at the phylotype level at time scales relevant to HNF duplication times. Omnivorous katablepharids and predatory MAST-2 dominated the HNF community, especially in spring. Bacterivorous groups (e.g., MAST-1, CRY1) were less abundant. Long-read sequencing revealed distinct seasonal shifts in dominant phylotypes, with Katablepharis sp. and MAST-2D peaking in spring, while other lineages became more prominent in summer and autumn. The high abundance of omnivorous HNF, compared to bacterivores, highlights their key role both as grazers of bacteria and flagellates, and as a food source for predatory and omnivorous ciliates.

Název v anglickém jazyce

High-temporal resolution of microbial food web dynamics and structure during phytoplankton blooms in the Baltic Sea

Popis výsledku anglicky

Heterotrophic nanoflagellates (HNF) are a key component of the microbial food webs, playing an essential role in nutrient recycling and energy transfer in aquatic ecosystems. They have been typically considered to be bacterivores, but they can also be omnivorous (feeding on prokaryotes and other eukaryotes) and predatory grazers (feeding on other eukaryotes). Here, we combine CARD-FISH with both short and long-amplicon sequencing to resolve the dynamics of key HNF groups during two high-frequency sampling campaigns in spring (March-May) and autumn (September-November) phytoplankton blooms in the coastal waters of the Baltic Sea. This approach allowed us to resolve the microbial food web dynamics within HNF communities at the phylotype level at time scales relevant to HNF duplication times. Omnivorous katablepharids and predatory MAST-2 dominated the HNF community, especially in spring. Bacterivorous groups (e.g., MAST-1, CRY1) were less abundant. Long-read sequencing revealed distinct seasonal shifts in dominant phylotypes, with Katablepharis sp. and MAST-2D peaking in spring, while other lineages became more prominent in summer and autumn. The high abundance of omnivorous HNF, compared to bacterivores, highlights their key role both as grazers of bacteria and flagellates, and as a food source for predatory and omnivorous ciliates.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/GF22-35826K" target="_blank" >GF22-35826K: Jak složité jsou mikrobiální potravní řetezce ve vodách?Objasnění role středně velkých heterotrofních bičíkovců ve sladkých a brakických habitatech</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology

ISSN

1462-2912

e-ISSN

1462-2920

Svazek periodika

27

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

e70190

Kód UT WoS článku

001605172700001

EID výsledku v databázi Scopus

2-s2.0-105020479988