Gradient patterns in the Tatra Mountain lakes revisited with invertebrate DNA metabarcoding

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00646963" target="_blank" >RIV/60077344:_____/25:00646963 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3897/mbmg.9.150825" target="_blank" >https://doi.org/10.3897/mbmg.9.150825</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3897/mbmg.9.150825" target="_blank" >10.3897/mbmg.9.150825</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gradient patterns in the Tatra Mountain lakes revisited with invertebrate DNA metabarcoding

Popis výsledku v původním jazyce

Monitoring changes in freshwater fauna requires timely, efficient, and comprehensive data collection, needs that are increasingly met by molecular methods. This is especially important in remote regions along environmental gradients, such as the Tatra Mountain lakes in Slovakia. In this study, DNA metabarcoding was applied to littoral benthic invertebrates collected from lakes in three valleys, spanning a gradient from the subalpine to alpine zones. Ordination analyses grouped the lakes based on community composition, and these groups were evaluated in relation to altitude, physico-chemical properties, indicator species, and previous data. To assess their additional value, surface sediment samples from the littoral zone were also analyzed using metabarcoding. The results revealed two distinct lake groups, primarily differentiated by altitude, alkalinity, and other correlated environmental parameters. The placement of specific lakes within these groups corresponded with known patterns of recovery from acidification, catchment characteristics, and potentially climate change or lake connectivity. Metabarcoding-based indicator species analysis successfully identified even taxa that had been previously overlooked or misidentified in morphology-based studies. While sediment metabarcoding provided limited supplementary information, its ordination patterns aligned with those from invertebrate analyses. Overall, our study of an alpine lake system supports broader trends: species richness declines with increasing elevation., community composition reflects environmental gradients., and DNA metabarcoding offers high taxonomic resolution and valuable insights into freshwater invertebrate biodiversity.

Název v anglickém jazyce

Gradient patterns in the Tatra Mountain lakes revisited with invertebrate DNA metabarcoding

Popis výsledku anglicky

Monitoring changes in freshwater fauna requires timely, efficient, and comprehensive data collection, needs that are increasingly met by molecular methods. This is especially important in remote regions along environmental gradients, such as the Tatra Mountain lakes in Slovakia. In this study, DNA metabarcoding was applied to littoral benthic invertebrates collected from lakes in three valleys, spanning a gradient from the subalpine to alpine zones. Ordination analyses grouped the lakes based on community composition, and these groups were evaluated in relation to altitude, physico-chemical properties, indicator species, and previous data. To assess their additional value, surface sediment samples from the littoral zone were also analyzed using metabarcoding. The results revealed two distinct lake groups, primarily differentiated by altitude, alkalinity, and other correlated environmental parameters. The placement of specific lakes within these groups corresponded with known patterns of recovery from acidification, catchment characteristics, and potentially climate change or lake connectivity. Metabarcoding-based indicator species analysis successfully identified even taxa that had been previously overlooked or misidentified in morphology-based studies. While sediment metabarcoding provided limited supplementary information, its ordination patterns aligned with those from invertebrate analyses. Overall, our study of an alpine lake system supports broader trends: species richness declines with increasing elevation., community composition reflects environmental gradients., and DNA metabarcoding offers high taxonomic resolution and valuable insights into freshwater invertebrate biodiversity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/GA23-06379S" target="_blank" >GA23-06379S: Horské ekosystémy na výškovém gradientu pod vlivem změn klimatických podmínek</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Metabarcoding and Metagenomics

ISSN

2534-9708

e-ISSN

2534-9708

Svazek periodika

9

Číslo periodika v rámci svazku

Jul

Stát vydavatele periodika

BG - Bulharská republika

Počet stran výsledku

21

Strana od-do

315-335

Kód UT WoS článku

001546426600001

EID výsledku v databázi Scopus

2-s2.0-105014620830