Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077387%3A_____%2F01%3A62013084" target="_blank" >RIV/60077387:_____/01:62013084 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
Popis výsledku v původním jazyce
Direct inhibiting effects of Al on extracellular phosphatases produced by the plankton were studied in lab experiments. Significant reduction of an apparent substrate affinity was found at Al concentrations 300-1000 g l<sup>-1</sup> at pH 4.5 and 4.8, while maximum acid phosphatase activity (APA) remained unchanged. Such behaviour of saturation kinetics is compatible with the assumption that ionic Al acts as a competitive inhibitor. To decide whether the observed Al effects could be explained alternatively by complexation of Al with substrate, the best fits of data with both possible models was statistically tested. Experimental results supported the competitive hypothesis rather than the complexation model. For pH values <5.2, the results of an Al-pHmatrix experiment gave a more detailed picture: the higher the Al concentration, the wider was the pH range where Al could negatively affect APA. The ecological ramifications of this effect were evaluated in the context of field APA data
Název v anglickém jazyce
Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
Popis výsledku anglicky
Direct inhibiting effects of Al on extracellular phosphatases produced by the plankton were studied in lab experiments. Significant reduction of an apparent substrate affinity was found at Al concentrations 300-1000 g l<sup>-1</sup> at pH 4.5 and 4.8, while maximum acid phosphatase activity (APA) remained unchanged. Such behaviour of saturation kinetics is compatible with the assumption that ionic Al acts as a competitive inhibitor. To decide whether the observed Al effects could be explained alternatively by complexation of Al with substrate, the best fits of data with both possible models was statistically tested. Experimental results supported the competitive hypothesis rather than the complexation model. For pH values <5.2, the results of an Al-pHmatrix experiment gave a more detailed picture: the higher the Al concentration, the wider was the pH range where Al could negatively affect APA. The ecological ramifications of this effect were evaluated in the context of field APA data
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DA - Hydrologie a limnologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2001
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Environmental Microbiology
ISSN
1462-2912
e-ISSN
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Svazek periodika
3
Číslo periodika v rámci svazku
9
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
10
Strana od-do
578-587
Kód UT WoS článku
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EID výsledku v databázi Scopus
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