Phagocytosis of Acanthamoeba sp. from aquifer of the Valley of Mezquital (State of Hidalgo, Mexico).

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phagocytosis of Acanthamoeba sp. from aquifer of the Valley of Mezquital (State of Hidalgo, Mexico).

Popis výsledku v původním jazyce

Genus Acanthamoeba dominated among the amoebae isolated from underground waters in the Valley of Mezquital (State of Hidalgo, Mexico). Feeding activity of Acanthamoeba sp. xenic isolate was studied in micro-well plate experiments using fluorescently labelled bacteria (FLB) method. A dispersed bacterial prey (Enterobacter aerogenes) was applied at a concentration up 10^7 FLB ml-1. Within the incubation (less than 1 h), amoebae ingested in an average 2 to 4 FLB cell-1, i.e., uptake rates of 6 to 54 FLB cell-1 h-1 and 1 to 12 vac cell-1 h-1 were reached, respectively. Generally, ingestion of FLB dropped due to an apparent saturation within the first 10 to 30 min of incubation with FLB. Numbers of FLB collected by amoebae from a dispersion varied too muchto state a feeding rate valid for all populations, however, the data have proven that acanthamoebae are able to collect efficiently a bacterial food from the dispersion at concentrations higher than 10^6 cells ml-1.

Název v anglickém jazyce

Phagocytosis of Acanthamoeba sp. from aquifer of the Valley of Mezquital (State of Hidalgo, Mexico).

Popis výsledku anglicky

Genus Acanthamoeba dominated among the amoebae isolated from underground waters in the Valley of Mezquital (State of Hidalgo, Mexico). Feeding activity of Acanthamoeba sp. xenic isolate was studied in micro-well plate experiments using fluorescently labelled bacteria (FLB) method. A dispersed bacterial prey (Enterobacter aerogenes) was applied at a concentration up 10^7 FLB ml-1. Within the incubation (less than 1 h), amoebae ingested in an average 2 to 4 FLB cell-1, i.e., uptake rates of 6 to 54 FLB cell-1 h-1 and 1 to 12 vac cell-1 h-1 were reached, respectively. Generally, ingestion of FLB dropped due to an apparent saturation within the first 10 to 30 min of incubation with FLB. Numbers of FLB collected by amoebae from a dispersion varied too muchto state a feeding rate valid for all populations, however, the data have proven that acanthamoebae are able to collect efficiently a bacterial food from the dispersion at concentrations higher than 10^6 cells ml-1.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Xth International Meeting on the Biology and Pathogenicity of Free-Living Amoebae, Proceedings.

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

129-136

Název nakladatele

Publicación Científica del Instituto Tecnológico

Místo vydání

Sonora

Místo konání akce

Sonora [MX]

Datum konání akce

5. 10. 2002

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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