Izolace a fúze mezofylových protoplastů bramboru elektrickým polem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F03%3A00010414" target="_blank" >RIV/60109807:_____/03:00010414 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Potato mesophyll protoplast isolation and protoplast fusion using electric field

Popis výsledku v původním jazyce

Mesophyll protoplasts were isolated from leaves of primary potato dihaploids, potato tetraploid of variety Bintje and from leaves of wild potato species S. brevidens (EBN = 1, 2n=24), S. bulbocastanum (EBN = 1, 2n=24). Donor plants were cultured on SH medium in vitro supplemented with 5 mg/l AgNO3 and 3 mg/l Alar 85 and 1,5% sucrose. Protoplasts were isolated in ezymatic mixture of 1% Cellulase Onozuka R-10 and 0,2% Macerozyme R-10 (or 2/3 of this concentration). Refined protoplasts were cultured on medium by Sidorov (1987), modified by Briza and Machova (1991). Conditions of electrofusion were optimized for protoplasts. Alternative current of 5 V was used for protoplast alignment with manual control of length of AC field activity. 1 pulse of direct current of 10 V and length of 80 us was used for the protoplast fusion. Protoplast viability was evaluated using FDA. In many the combinations tested, cell wall regeneration and in several cases microcolony formation was observed after elec

Název v anglickém jazyce

Potato mesophyll protoplast isolation and protoplast fusion using electric field

Popis výsledku anglicky

Mesophyll protoplasts were isolated from leaves of primary potato dihaploids, potato tetraploid of variety Bintje and from leaves of wild potato species S. brevidens (EBN = 1, 2n=24), S. bulbocastanum (EBN = 1, 2n=24). Donor plants were cultured on SH medium in vitro supplemented with 5 mg/l AgNO3 and 3 mg/l Alar 85 and 1,5% sucrose. Protoplasts were isolated in ezymatic mixture of 1% Cellulase Onozuka R-10 and 0,2% Macerozyme R-10 (or 2/3 of this concentration). Refined protoplasts were cultured on medium by Sidorov (1987), modified by Briza and Machova (1991). Conditions of electrofusion were optimized for protoplasts. Alternative current of 5 V was used for protoplast alignment with manual control of length of AC field activity. 1 pulse of direct current of 10 V and length of 80 us was used for the protoplast fusion. Protoplast viability was evaluated using FDA. In many the combinations tested, cell wall regeneration and in several cases microcolony formation was observed after elec

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/OC%20843.20" target="_blank" >OC 843.20: Somatická hybridizace ve šlechtění bramboru</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Materialy Meždunarodnoj jubilejnoj naučno-praktičeskoj konferencii posvjaščennoj 75-letiju Instituta kartofelevodstva Nacionalnoj akademii nauk Belarusi

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

337-340

Název nakladatele

Merlit

Místo vydání

Minsk

Místo konání akce

Samochvaloviči

Datum konání akce

7. 7. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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