Biolistický přenos populací viroidu vřetenovitosti hlíz bramboru (PSTVd) do plevelných rostlin vyskytujících se na bramborovýc polích a patogeneze PSTVd na Chamomilla reculita

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F08%3A%230000099" target="_blank" >RIV/60109807:_____/08:#0000099 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biolistic Transmission of potato spindle tuber viroid (PSTVd) Populations to Weeds frequently grown on Potato Fields and PSTVd pathogenesis on Cultured Chamomilla reculita

Popis výsledku v původním jazyce

Weed plants characteristic for potato fields have not been considered in the past as potential hosts that could transmit and lead to spreading of potato spindle tuber viroid (PSTVd). To gain insight into this problem, we biolistically inoculated these weed plants with viroid populations either as RNA or as cDNA PSTVd variants. New potential viroid host species, collected in central Europe, were discovered. From 12 weed species characteristic for potato fields, high viroid levels, detectable by molecularhybridization, were maintained after both RNA and DNA transfers in Chamomilla reculita and Anthemis arvensis. Low viroid levels, detectable by reverse transcription-PCR (RT-PCR) only, were maintained after plant inoculations with cDNA in Veronica argensis and Amaranthus retroflexus. In cultured chamomille (Chamomilla reculita var.Bohemia) the pathogenesis symptoms were observed in similar strength as for tomato cv. Rutgers. The strongest symptoms were characteristic for letal strain AS1

Název v anglickém jazyce

Biolistic Transmission of potato spindle tuber viroid (PSTVd) Populations to Weeds frequently grown on Potato Fields and PSTVd pathogenesis on Cultured Chamomilla reculita

Popis výsledku anglicky

Weed plants characteristic for potato fields have not been considered in the past as potential hosts that could transmit and lead to spreading of potato spindle tuber viroid (PSTVd). To gain insight into this problem, we biolistically inoculated these weed plants with viroid populations either as RNA or as cDNA PSTVd variants. New potential viroid host species, collected in central Europe, were discovered. From 12 weed species characteristic for potato fields, high viroid levels, detectable by molecularhybridization, were maintained after both RNA and DNA transfers in Chamomilla reculita and Anthemis arvensis. Low viroid levels, detectable by reverse transcription-PCR (RT-PCR) only, were maintained after plant inoculations with cDNA in Veronica argensis and Amaranthus retroflexus. In cultured chamomille (Chamomilla reculita var.Bohemia) the pathogenesis symptoms were observed in similar strength as for tomato cv. Rutgers. The strongest symptoms were characteristic for letal strain AS1

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vědecké práce VÚB 16

ISSN

1802-940X

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

2008

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

13

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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