Intra- and inter-specific crosses of Solanum materials after mitotic polyploidization in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F09%3A0R000265" target="_blank" >RIV/60109807:_____/09:0R000265 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Intra- and inter-specific crosses of Solanum materials after mitotic polyploidization in vitro

Popis výsledku v původním jazyce

Mitotic polyploidization in vitro was used in selected wild Solanum species and S.tuberosum dihaploids. Oryzalin was more effective than colchicine (P = 0.1). The rate of non-affected to mixoploid to tetraploid regenerants was 22 : 2.5 : 1 (colchicine) and 14 : 2 : 1 (oryzalin). Optimal concentrations and durations were 3.5 mm/24 h for colchicine and 25 or 30 mu m for 24 or 48 h for oryzalin (variations in concentration and duration are necessary owing to possible diversity of responses in selected genotypes). Tetraploids were obtained from S. berthaultii, S. bulbocastanum, S. pinnatisectum, S. verrucosum and eleven S. tuberosum dihaploids. The yield of tetraploids derived from tbr dihaploids was lower than that from the wild species (P = 0.01). Tetraploid regenerants were tested in intra- and inter-specific crosses. Three of 43 intra-specific combinations were successful and yielded 440 seeds. Inter-specific crosses yielded 48 seedless berries.

Název v anglickém jazyce

Intra- and inter-specific crosses of Solanum materials after mitotic polyploidization in vitro

Popis výsledku anglicky

Mitotic polyploidization in vitro was used in selected wild Solanum species and S.tuberosum dihaploids. Oryzalin was more effective than colchicine (P = 0.1). The rate of non-affected to mixoploid to tetraploid regenerants was 22 : 2.5 : 1 (colchicine) and 14 : 2 : 1 (oryzalin). Optimal concentrations and durations were 3.5 mm/24 h for colchicine and 25 or 30 mu m for 24 or 48 h for oryzalin (variations in concentration and duration are necessary owing to possible diversity of responses in selected genotypes). Tetraploids were obtained from S. berthaultii, S. bulbocastanum, S. pinnatisectum, S. verrucosum and eleven S. tuberosum dihaploids. The yield of tetraploids derived from tbr dihaploids was lower than that from the wild species (P = 0.01). Tetraploid regenerants were tested in intra- and inter-specific crosses. Three of 43 intra-specific combinations were successful and yielded 440 seeds. Inter-specific crosses yielded 48 seedless berries.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QF4133" target="_blank" >QF4133: Tvorba výchozích šlechtitelských materiálů s geny horizontální rezistence k plísni bramborové</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT BREEDING

ISSN

0179-9541

e-ISSN

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Svazek periodika

128

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000272131400015

EID výsledku v databázi Scopus

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