CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F09%3A0R000947" target="_blank" >RIV/60109807:_____/09:0R000947 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS

Popis výsledku v původním jazyce

136 diploid genotypes of genus Solanum and diploid potato were selected and characterised as the potential resources of resistance genes against potato late blight. For a consideration of infectious tests by inoculation of leaf discs and in vitro plants,15 highly resistant genotypes of wild species were selected; 6 of them showed no symptoms. In contrast, all 11 tested diploids of Solanum tuberosum were susceptible. Finally there were selected and characterised in detail 20 clones (10 clones of Solanumbulbocastanum, 1 clone of Solanum verrucosum and 9 clones of diploid Solanum tuberosum) that were useful for somatic hybridisation by electrofusion. The protoplast yield ranged between 1 x 105 and 3,5 x 106 per ml from on average 325 mg of plant leaf material and it was variable within single repetitions of one genotype too. The positive correlation between yield and vitality of isolated protoplasts was detected, r = 0.476 (mesoscale dependence).

Název v anglickém jazyce

CHARACTERIZATION OF SELECTED DIPLOID GENETIC RESOURCES OF GENUS SOLANUM INTENDED FOR SOMATIC HYBRIDIZATION WITH POTATO DIHAPLOIDS

Popis výsledku anglicky

136 diploid genotypes of genus Solanum and diploid potato were selected and characterised as the potential resources of resistance genes against potato late blight. For a consideration of infectious tests by inoculation of leaf discs and in vitro plants,15 highly resistant genotypes of wild species were selected; 6 of them showed no symptoms. In contrast, all 11 tested diploids of Solanum tuberosum were susceptible. Finally there were selected and characterised in detail 20 clones (10 clones of Solanumbulbocastanum, 1 clone of Solanum verrucosum and 9 clones of diploid Solanum tuberosum) that were useful for somatic hybridisation by electrofusion. The protoplast yield ranged between 1 x 105 and 3,5 x 106 per ml from on average 325 mg of plant leaf material and it was variable within single repetitions of one genotype too. The positive correlation between yield and vitality of isolated protoplasts was detected, r = 0.476 (mesoscale dependence).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QF4107" target="_blank" >QF4107: Vývoj metod identifikace a charakterizace donorů rezistence plísně bramborové v genofondu bramboru pomocí DNA markerů a biologického testu v in vitro podmínkách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Polnohospodarstvo

ISSN

0551-3677

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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