The utilization of molecular markers in recent years at selected interspecific hybrids of Solanum species.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F24%3AN0000007" target="_blank" >RIV/60109807:_____/24:N0000007 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The utilization of molecular markers in recent years at selected interspecific hybrids of Solanum species.

Popis výsledku v původním jazyce

The selected wild Solanum species were chosen for crossing by means of traditional breeding methods. These wild species, maintained in genebank of Potato Research Institute in Havlíčkův Brod, are a good source of resistance to various biotic and abiotic factors. Hundreds of hybrids from many combinations, which were arisen by reciprocal crossing procedures (wild species x variety or dihaploid) and parental genotypes were tested by means of PCR methods. We obtained the crossing materials with wild species (S. acaule, S. berthaultii and S. demissum) with potato varieties. Hybridity of progeny after interspecific hybridization was visually checked by morphological traits and RAPD method. The hybridity was found at some hybrids from crossing combinations only. By means of PCR genotypes from seven wild species (S. acaule, S. berthaultii, S. bulbocastanum, S. brevidens, S. demissum, S. pinnatisectum and S. verrucosum) were tested. The plant material was transferred from in vitro conditions to the greenhouse and grown there. The DNA was isolated from these plants by means of GenElute Plant Genomic DNA Miniprep Kit (Sigma). DNA markers were chosen and tested. The selected markers were used for subsequent testing of diploid and tetraploid potato collections. The wild species bearing rresistance genes are used for crossing experiments. The very precise genomic sequence allows for faster and more targeted breeding, as it is easier to find in DNA which crosses with other varieties might be of interest and where the exchange of genetic material between "father" and "mother" should ideally take place. This means that breeders know at an early stage whether a potato has the required properties, such as resistance to specific diseases.

Název v anglickém jazyce

The utilization of molecular markers in recent years at selected interspecific hybrids of Solanum species.

Popis výsledku anglicky

The selected wild Solanum species were chosen for crossing by means of traditional breeding methods. These wild species, maintained in genebank of Potato Research Institute in Havlíčkův Brod, are a good source of resistance to various biotic and abiotic factors. Hundreds of hybrids from many combinations, which were arisen by reciprocal crossing procedures (wild species x variety or dihaploid) and parental genotypes were tested by means of PCR methods. We obtained the crossing materials with wild species (S. acaule, S. berthaultii and S. demissum) with potato varieties. Hybridity of progeny after interspecific hybridization was visually checked by morphological traits and RAPD method. The hybridity was found at some hybrids from crossing combinations only. By means of PCR genotypes from seven wild species (S. acaule, S. berthaultii, S. bulbocastanum, S. brevidens, S. demissum, S. pinnatisectum and S. verrucosum) were tested. The plant material was transferred from in vitro conditions to the greenhouse and grown there. The DNA was isolated from these plants by means of GenElute Plant Genomic DNA Miniprep Kit (Sigma). DNA markers were chosen and tested. The selected markers were used for subsequent testing of diploid and tetraploid potato collections. The wild species bearing rresistance genes are used for crossing experiments. The very precise genomic sequence allows for faster and more targeted breeding, as it is easier to find in DNA which crosses with other varieties might be of interest and where the exchange of genetic material between "father" and "mother" should ideally take place. This means that breeders know at an early stage whether a potato has the required properties, such as resistance to specific diseases.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Vědecké práce VÚB Havlíčkův Brod

ISSN

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e-ISSN

3029-7702

Svazek periodika

30

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

9-18

Kód UT WoS článku

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EID výsledku v databázi Scopus

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