Proteomics approach in classifying the biochemical basis of the anticancer activity of the new olomoucine-derived synthetic cyclin-dependent kinase inhibitor, bohemine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG16__%2F00%3A00000359" target="_blank" >RIV/60162694:G16__/00:00000359 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/00:00001323 RIV/60162694:G38__/00:00000359

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Proteomics approach in classifying the biochemical basis of the anticancer activity of the new olomoucine-derived synthetic cyclin-dependent kinase inhibitor, bohemine

Popis výsledku v původním jazyce

In eukaryotic cells the activity of cyclin dependent kinases (CDKs) is precisely regulated. This regulation maintains a proper coordination of the individual steps in cell division cycle. Due to the fact that the family of natural CDK inhibitors (CDKIs)belong to the most frequently mutated proteins in cancer cells, molecules that could mimic their biological activities are attractive candidates for anti-cancer treatment. The aim of this study was use 2-DE coupled with multivariate principal component analysis (PCA) to characterize the quantitative changes in the protein composition of the CEM T-lymphoblastic leukemia cell line after treatment with bohemine (BOH), a synthetic olomoucin derived CDKI. Cell classification, reflecting protein patterns,clearly distinguished two main groups: one group consists of 9, 12 and 24 - hour treated BOH cells while the second is represented by the 0 and 24 - hour control untreated cells and the 6- hour BOH exposed CEM lymphoblasts. Discriminant prote

Název v anglickém jazyce

Proteomics approach in classifying the biochemical basis of the anticancer activity of the new olomoucine-derived synthetic cyclin-dependent kinase inhibitor, bohemine

Popis výsledku anglicky

In eukaryotic cells the activity of cyclin dependent kinases (CDKs) is precisely regulated. This regulation maintains a proper coordination of the individual steps in cell division cycle. Due to the fact that the family of natural CDK inhibitors (CDKIs)belong to the most frequently mutated proteins in cancer cells, molecules that could mimic their biological activities are attractive candidates for anti-cancer treatment. The aim of this study was use 2-DE coupled with multivariate principal component analysis (PCA) to characterize the quantitative changes in the protein composition of the CEM T-lymphoblastic leukemia cell line after treatment with bohemine (BOH), a synthetic olomoucin derived CDKI. Cell classification, reflecting protein patterns,clearly distinguished two main groups: one group consists of 9, 12 and 24 - hour treated BOH cells while the second is represented by the 0 and 24 - hour control untreated cells and the 6- hour BOH exposed CEM lymphoblasts. Discriminant prote

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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