Idarubicin - Induced apoptosis in HL-60 cells: relationship between apoptosis and time dependence of apoptosis on Idarubicin dose

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG16__%2F00%3A00000377" target="_blank" >RIV/60162694:G16__/00:00000377 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Idarubicin - Induced apoptosis in HL-60 cells: relationship between apoptosis and time dependence of apoptosis on Idarubicin dose

Popis výsledku v původním jazyce

Apoptosis in HL-60 human leukaemia cells treated in vitro with idarubicin (4 hours incubation) was quantified using morphologic changes in the nuclear and cytoplasmic structures in Diff-Quik stained cytospin preparations, flow cytometric measurement of sub-diploid DNA content (sub-G1 cells) and increase of staining with APO 2.7 antibody in nonpermeabilised cells. After treatment by high (20-100 nmol) doses of idarubicin HL-60 cells were found to die earlier, primarily by apoptosis, regardless of the cell-cycle phase, in which they were affected. We have observed that after 6 hours of incubation with small (10 nmol/l) idarubicin dose most of the live cells were in S phase of cell cycle (62%), after 24 hour most of them moved to G2 phase (61%). Increaseof apoptotic cells (20%) was observed as late as 48 and 72 hours after 4 hours incubation with idarubicin. After 72 hours 50% of cells were necrotic (Trypan blue staining).

Název v anglickém jazyce

Idarubicin - Induced apoptosis in HL-60 cells: relationship between apoptosis and time dependence of apoptosis on Idarubicin dose

Popis výsledku anglicky

Apoptosis in HL-60 human leukaemia cells treated in vitro with idarubicin (4 hours incubation) was quantified using morphologic changes in the nuclear and cytoplasmic structures in Diff-Quik stained cytospin preparations, flow cytometric measurement of sub-diploid DNA content (sub-G1 cells) and increase of staining with APO 2.7 antibody in nonpermeabilised cells. After treatment by high (20-100 nmol) doses of idarubicin HL-60 cells were found to die earlier, primarily by apoptosis, regardless of the cell-cycle phase, in which they were affected. We have observed that after 6 hours of incubation with small (10 nmol/l) idarubicin dose most of the live cells were in S phase of cell cycle (62%), after 24 hour most of them moved to G2 phase (61%). Increaseof apoptotic cells (20%) was observed as late as 48 and 72 hours after 4 hours incubation with idarubicin. After 72 hours 50% of cells were necrotic (Trypan blue staining).

Druh

C - Kapitola v odborné knize

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Cells II

ISBN

80-7232-120-X

Počet stran výsledku

1

Strana od-do

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Počet stran knihy

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Název nakladatele

Kopp

Místo vydání

České Budějovice

Kód UT WoS kapitoly

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