Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG42__%2F21%3A00556954" target="_blank" >RIV/60162694:G42__/21:00556954 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/22:63529198 RIV/70883521:28610/22:63529198

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s13197-021-05090-9" target="_blank" >https://link.springer.com/article/10.1007/s13197-021-05090-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13197-021-05090-9" target="_blank" >10.1007/s13197-021-05090-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR

Popis výsledku v původním jazyce

Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we design two sets of primers for the detection and quantification of the amine oxidase gene (yobN) and endogenous (housekeeping) gene (gyrB) in Bacillus subtilis. Moreover, these sets can be used for relative quantification of yobN by real-time PCR (qPCR). We also tested the degradation of BAs by three bacterial strains (B. subtilis strains: IB1a, CCM 2216, CCM 2267) in a mineral medium over a two-day period. Their degradation abilities were verified by high performance liquid chromatography with UV detection (HPLC/UV). According to the results, two strains significantly (P < 0.05) reduced histamine, tyramine, putrescine, and cadaverine. Moreover, our results indicate that the degradation ability of B. subtilis strains could be limited by sporulation because the gene encoding amine oxidase (yobN) is no longer expressed in the spores.

Název v anglickém jazyce

Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR

Popis výsledku anglicky

Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we design two sets of primers for the detection and quantification of the amine oxidase gene (yobN) and endogenous (housekeeping) gene (gyrB) in Bacillus subtilis. Moreover, these sets can be used for relative quantification of yobN by real-time PCR (qPCR). We also tested the degradation of BAs by three bacterial strains (B. subtilis strains: IB1a, CCM 2216, CCM 2267) in a mineral medium over a two-day period. Their degradation abilities were verified by high performance liquid chromatography with UV detection (HPLC/UV). According to the results, two strains significantly (P < 0.05) reduced histamine, tyramine, putrescine, and cadaverine. Moreover, our results indicate that the degradation ability of B. subtilis strains could be limited by sporulation because the gene encoding amine oxidase (yobN) is no longer expressed in the spores.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

<a href="/cs/project/GA17-09594S" target="_blank" >GA17-09594S: Redukce obsahu biogenních aminů v modelových systémech</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE

ISSN

0022-1155

e-ISSN

0975-8402

Svazek periodika

—

Číslo periodika v rámci svazku

58

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

8

Strana od-do

8

Kód UT WoS článku

000639073800002

EID výsledku v databázi Scopus

2-s2.0-85104147963