Interakce B buněk s intracelulárním patogenem Francisella tularensis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F08%3A00001937" target="_blank" >RIV/60162694:G44__/08:00001937 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of B cells with intracellular pathogen Francisella tularensis

Popis výsledku v původním jazyce

Immunity to Francisella tularensis is largely mediated by T lymphocytes but an important role of B lymphocytes in early stage of infection was previously uncovered. We wanted to find out if F. tularensis is able to infect B cells and/or influence them bydirect contact. To investigate this possibility we infected B cell lines from mouse (A20) or humans (Ramos RA-1), or primary mouse spleen cells, with F. tularensis LVS and F. tularensis FSC200 in vitro. In all cases, we infetected bacteria on the cell surface and inside the B cells using transmission electron microscopy. More than 20% cells were infected by microbes after 24 h. The number of bacteria, determined by CFU, increased about 1 log during 24 h. Infection with live bacteria led to apoptosis ofRamos cells and mouse CD19 spleen cells. Approximately 30% of cells were apoptotic after 24 h and 70% after 48 h, independently of the F. tularensis strain, while only 10% of noninfected cell were apoptotic at either time point. Apoptosi

Název v anglickém jazyce

Interaction of B cells with intracellular pathogen Francisella tularensis

Popis výsledku anglicky

Immunity to Francisella tularensis is largely mediated by T lymphocytes but an important role of B lymphocytes in early stage of infection was previously uncovered. We wanted to find out if F. tularensis is able to infect B cells and/or influence them bydirect contact. To investigate this possibility we infected B cell lines from mouse (A20) or humans (Ramos RA-1), or primary mouse spleen cells, with F. tularensis LVS and F. tularensis FSC200 in vitro. In all cases, we infetected bacteria on the cell surface and inside the B cells using transmission electron microscopy. More than 20% cells were infected by microbes after 24 h. The number of bacteria, determined by CFU, increased about 1 log during 24 h. Infection with live bacteria led to apoptosis ofRamos cells and mouse CD19 spleen cells. Approximately 30% of cells were apoptotic after 24 h and 70% after 48 h, independently of the F. tularensis strain, while only 10% of noninfected cell were apoptotic at either time point. Apoptosi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP310%2F06%2FP266" target="_blank" >GP310/06/P266: Proteomová analýza bakterie Francisella tularensis LVS kultivované intracelulárně in vitro a in vivo, identifikace potenciálních virulentních faktorů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Pathogenesis

ISSN

0882-4010

e-ISSN

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Svazek periodika

45

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000262591500001

EID výsledku v databázi Scopus

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