Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002534" target="_blank" >RIV/60162694:G44__/11:00002534 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/11:10105466

Výsledek na webu

<a href="http://dx.doi.org/10.2478/v10136-011-0007-2" target="_blank" >http://dx.doi.org/10.2478/v10136-011-0007-2</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats

Popis výsledku v původním jazyce

Lymphocytes are the most radiosensitive cells. After exposure IR die by apoptosis at a rate proportional to the dose received. We demonstrated that one hour after whole-body irradiation of rats, histone H2AX in the lymphocyte nuclei was quickly phosphorylated on serine 139. In the work presented here, we studied the kinetics of lymphocyte depletion in the blood and phosphorylation of histone H2AX in the peripheral blood lymphocytes after local (thoracic) irradiation of rats. Twenty-four hours after whole-body irradiation of the rats at a dose of 5 Gy, the lymphocyte count declined to almost zero values, whereas after local irradiation of the thorax area, the counts of lymphocytes in the peripheral blood remained unaltered. We employed flow-cytometric and microscopic gammaH2AX determination 1 h after thoracic irradiation of rats. We revealed a dose dependence on the increase in gammaH2AX in a dose range of 10-30 Gy. The microscopic method was more sensitive in the case of lower radiatio

Název v anglickém jazyce

Phosphorylation of histone H2AX in peripheral blood mononuclear cells after thoracic irradiation of rats

Popis výsledku anglicky

Lymphocytes are the most radiosensitive cells. After exposure IR die by apoptosis at a rate proportional to the dose received. We demonstrated that one hour after whole-body irradiation of rats, histone H2AX in the lymphocyte nuclei was quickly phosphorylated on serine 139. In the work presented here, we studied the kinetics of lymphocyte depletion in the blood and phosphorylation of histone H2AX in the peripheral blood lymphocytes after local (thoracic) irradiation of rats. Twenty-four hours after whole-body irradiation of the rats at a dose of 5 Gy, the lymphocyte count declined to almost zero values, whereas after local irradiation of the thorax area, the counts of lymphocytes in the peripheral blood remained unaltered. We employed flow-cytometric and microscopic gammaH2AX determination 1 h after thoracic irradiation of rats. We revealed a dose dependence on the increase in gammaH2AX in a dose range of 10-30 Gy. The microscopic method was more sensitive in the case of lower radiatio

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/OVUOFVZ200806" target="_blank" >OVUOFVZ200806: RONSDOZ - Neinvazivní stanovení prozánětlivých ukazatelů oxidačního stresu u ozářených jako indikátor obdržené dávky záření. Ochranný efekt acetyl-L-karnitinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Biomedicine - print

ISSN

1214-021X

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

209-218

Kód UT WoS článku

000295970900004

EID výsledku v databázi Scopus

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