Characterization of CD34(+) thymocytes in newborn dogs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F12%3A43874712" target="_blank" >RIV/60162694:G44__/12:43874712 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/12:#0000915

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetimm.2012.04.006" target="_blank" >http://dx.doi.org/10.1016/j.vetimm.2012.04.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetimm.2012.04.006" target="_blank" >10.1016/j.vetimm.2012.04.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of CD34(+) thymocytes in newborn dogs

Popis výsledku v původním jazyce

Using two-color flow cytometry, we characterized CD34(+) cells in the newborn canine thymus. CD34(+) thymic cells comprised approximately 5% of cells recovered by thymus tissue teasing and both large and small thymocytes have been present in this population, the former being 7-12 times more frequent. All CD34(+) cells expressed the pan-leukocyte antigen CD45. The expression of CD44 profile on the large and small CD34(+) thymocytes differed: almost all large CD34(+) cells were CD44(+), while only 75% ofsmall CD34(+) thymocytes co-expressed the CD44 antigen. We have previously described that CD172 alpha is present on the surface of CD34(+) bone marrow cells in dogs. In the thymus, CD172 alpha was expressed on 5-10% and less than 5% of large and small CD34(+) cells, respectively. Some CD34(+) thymocytes also co-expressed T-lineage-specific markers like CD3, CD4, CD8,TCR1 and TCR2. Their expression increased during the large-to-small thymocyte transition. Based on our findings we suggest

Název v anglickém jazyce

Characterization of CD34(+) thymocytes in newborn dogs

Popis výsledku anglicky

Using two-color flow cytometry, we characterized CD34(+) cells in the newborn canine thymus. CD34(+) thymic cells comprised approximately 5% of cells recovered by thymus tissue teasing and both large and small thymocytes have been present in this population, the former being 7-12 times more frequent. All CD34(+) cells expressed the pan-leukocyte antigen CD45. The expression of CD44 profile on the large and small CD34(+) thymocytes differed: almost all large CD34(+) cells were CD44(+), while only 75% ofsmall CD34(+) thymocytes co-expressed the CD44 antigen. We have previously described that CD172 alpha is present on the surface of CD34(+) bone marrow cells in dogs. In the thymus, CD172 alpha was expressed on 5-10% and less than 5% of large and small CD34(+) cells, respectively. Some CD34(+) thymocytes also co-expressed T-lineage-specific markers like CD3, CD4, CD8,TCR1 and TCR2. Their expression increased during the large-to-small thymocyte transition. Based on our findings we suggest

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Immunology and Immunopathology

ISSN

0165-2427

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

86-90

Kód UT WoS článku

000304687600010

EID výsledku v databázi Scopus

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