An acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F13%3A43874955" target="_blank" >RIV/60162694:G44__/13:43874955 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/13:PU126046

Výsledek na webu

<a href="http://www.mdpi.com/1424-8220/13/9/11498" target="_blank" >http://www.mdpi.com/1424-8220/13/9/11498</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/s130911498" target="_blank" >10.3390/s130911498</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents

Popis výsledku v původním jazyce

The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides,some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10-12 mol/L for sarin, 6.31 x 10-12 mol /L for soman, 6.17 x 10-11 mol/L for tabun, and 2.19 x 10-11 mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, met

Název v anglickém jazyce

An acetylcholinesterase based chronoamperometric biosensor for fast and reliable assay of nerve agents

Popis výsledku anglicky

The enzyme acetylcholinesterase (AChE) is an important part of cholinergic nervous system, where it stops neurotransmission by hydrolysis of the neurotransmitter acetylcholine. It is sensitive to inhibition by organophosphate and carbamate insecticides,some Alzheimer disease drugs, secondary metabolites such as aflatoxins and nerve agents used in chemical warfare. When immobilized on a sensor (physico-chemical transducer), it can be used for assay of these inhibitors. In the experiments described herein, an AChE- based electrochemical biosensor using screen printed electrode systems was prepared. The biosensor was used for assay of nerve agents such as sarin, soman, tabun and VX. The limits of detection achieved in a measuring protocol lasting ten minutes were 7.41 x 10-12 mol/L for sarin, 6.31 x 10-12 mol /L for soman, 6.17 x 10-11 mol/L for tabun, and 2.19 x 10-11 mol/L for VX, respectively. The assay was reliable, with minor interferences caused by the organic solvents ethanol, met

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

KA - Vojenství

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors

ISSN

1424-8220

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

11498-11506

Kód UT WoS článku

000328625800021

EID výsledku v databázi Scopus

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