Copper, aluminum, iron and calcium inhibit human acetylcholinesterase in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F14%3A43875538" target="_blank" >RIV/60162694:G44__/14:43875538 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S1382668914000027" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1382668914000027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.etap.2014.01.001" target="_blank" >10.1016/j.etap.2014.01.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Copper, aluminum, iron and calcium inhibit human acetylcholinesterase in vitro

Popis výsledku v původním jazyce

Acetylcholinesterase (AChE) is an important part of cholinergic nerves where it participates in termination of neurotransmission. AChE can be inhibited by e.g. some Alzheimer disease drugs, nerve agents, and secondary metabolites. In this work, metal salts aluminium chloride, calcium chloride, cupric chloride, ferric chloride, potassium chloride, magnesium chloride and sodium chloride were tested for their ability to inhibit AChE. Standard Ellman assay based on human recombinant AChE was done and inhibition was measured using Dixon plot. No inhibition was proved for sodium, potassium and magnesium ions. However, aluminium, cupric, ferric and calcium ions were able to inhibit AChE via noncompetitive mechanism of inhibition. Though the inhibition is muchweaker when compared to e.g. drugs with noncompetitive mechanism of action, biological relevance of the findings can be anticipated.

Název v anglickém jazyce

Copper, aluminum, iron and calcium inhibit human acetylcholinesterase in vitro

Popis výsledku anglicky

Acetylcholinesterase (AChE) is an important part of cholinergic nerves where it participates in termination of neurotransmission. AChE can be inhibited by e.g. some Alzheimer disease drugs, nerve agents, and secondary metabolites. In this work, metal salts aluminium chloride, calcium chloride, cupric chloride, ferric chloride, potassium chloride, magnesium chloride and sodium chloride were tested for their ability to inhibit AChE. Standard Ellman assay based on human recombinant AChE was done and inhibition was measured using Dixon plot. No inhibition was proved for sodium, potassium and magnesium ions. However, aluminium, cupric, ferric and calcium ions were able to inhibit AChE via noncompetitive mechanism of inhibition. Though the inhibition is muchweaker when compared to e.g. drugs with noncompetitive mechanism of action, biological relevance of the findings can be anticipated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LH11023" target="_blank" >LH11023: Vylepšení vakcinační účinnosti pomocí cholinergní protizánětlivé dráhy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Toxicology and Pharmacology

ISSN

1382-6689

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

455-459

Kód UT WoS článku

000332919800051

EID výsledku v databázi Scopus

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