Electrochemical determination of activity of acetylcholinesterase immobilized on magnetic particles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875609" target="_blank" >RIV/60162694:G44__/16:43875609 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/16:43909873 RIV/00216275:25310/16:39902006

Výsledek na webu

<a href="http://electrochemsci.org/papers/vol11/110604840.pdf" target="_blank" >http://electrochemsci.org/papers/vol11/110604840.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.20964/2016.06.39" target="_blank" >10.20964/2016.06.39</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical determination of activity of acetylcholinesterase immobilized on magnetic particles

Popis výsledku v původním jazyce

Acetylcholinesterase (AChE) is an enzyme which terminates action of neurotransmitter acetylcholine in cholinergic system. Activity of enzyme is commonly assayed by Ellman's method which has significant disadvantages like instability of reagents. Inability to use enzyme from the mixture repeatedly is another disadvantage. Therefore, we developed process using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) to immobilize AChE on the surface of magnetic particles, which enable to separate enzyme and use it repeatedly. To determinate activity of enzyme, we optimized square wave voltammetry (SWV) method where screen-printed sensor with Prussian blue (PB) modified working electrode was performed. We report limit of detection equal 8.1 mu M for a tested inhibitor of AChE tacrine. Comparing to the standard Ellman's test, the immobilized enzyme has limited sensitivity to interferences caused by organic solvents. The proposed method is readily to practical application.

Název v anglickém jazyce

Electrochemical determination of activity of acetylcholinesterase immobilized on magnetic particles

Popis výsledku anglicky

Acetylcholinesterase (AChE) is an enzyme which terminates action of neurotransmitter acetylcholine in cholinergic system. Activity of enzyme is commonly assayed by Ellman's method which has significant disadvantages like instability of reagents. Inability to use enzyme from the mixture repeatedly is another disadvantage. Therefore, we developed process using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) to immobilize AChE on the surface of magnetic particles, which enable to separate enzyme and use it repeatedly. To determinate activity of enzyme, we optimized square wave voltammetry (SWV) method where screen-printed sensor with Prussian blue (PB) modified working electrode was performed. We report limit of detection equal 8.1 mu M for a tested inhibitor of AChE tacrine. Comparing to the standard Ellman's test, the immobilized enzyme has limited sensitivity to interferences caused by organic solvents. The proposed method is readily to practical application.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

10

Strana od-do

4840-4849

Kód UT WoS článku

000378559300050

EID výsledku v databázi Scopus

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