Acetylcholinesterase inhibitors assay using colorimetric pH sensitive strips and image analysis by a smartphone

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F17%3A43875731" target="_blank" >RIV/60162694:G44__/17:43875731 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/17:43913005 RIV/00216275:25310/17:39902674

Výsledek na webu

<a href="https://www.hindawi.com/journals/ijac/2017/3712384/" target="_blank" >https://www.hindawi.com/journals/ijac/2017/3712384/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2017/3712384" target="_blank" >10.1155/2017/3712384</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acetylcholinesterase inhibitors assay using colorimetric pH sensitive strips and image analysis by a smartphone

Popis výsledku v původním jazyce

Smartphones are widely spread and their usage does not require any trained personnel. Recently, smartphones were successfully used in analytical chemistry as a simple detection tool in some applications. This paper focuses on immobilization of acetylcholinesterase (AChE) onto commercially available pH strips with stabilization in the gelatin membrane. AChE degrades acetylcholine into choline and acetic acid which causes color change of acid-base indicator. Smartphone served as a tool for measurement of indicator color change from red to orange while inhibitors blocked this process. AChE inhibitors were measured with limits of detection, 149 nM and 22.3 nM for galanthamine and donepezil, respectively. Organic solvents were measured for method interferences. Measurement procedure was performed on 3D printed holder and digital photography was evaluated using red-green-blue (RGB) channels. The invented assay was validated to the standard Ellman&apos;s test and verified on murine plasma samples spiked with inhibitors. We consider that the assay is fully suitable for practical performance.

Název v anglickém jazyce

Acetylcholinesterase inhibitors assay using colorimetric pH sensitive strips and image analysis by a smartphone

Popis výsledku anglicky

Smartphones are widely spread and their usage does not require any trained personnel. Recently, smartphones were successfully used in analytical chemistry as a simple detection tool in some applications. This paper focuses on immobilization of acetylcholinesterase (AChE) onto commercially available pH strips with stabilization in the gelatin membrane. AChE degrades acetylcholine into choline and acetic acid which causes color change of acid-base indicator. Smartphone served as a tool for measurement of indicator color change from red to orange while inhibitors blocked this process. AChE inhibitors were measured with limits of detection, 149 nM and 22.3 nM for galanthamine and donepezil, respectively. Organic solvents were measured for method interferences. Measurement procedure was performed on 3D printed holder and digital photography was evaluated using red-green-blue (RGB) channels. The invented assay was validated to the standard Ellman&apos;s test and verified on murine plasma samples spiked with inhibitors. We consider that the assay is fully suitable for practical performance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30107 - Medicinal chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Analytical Chemistry

ISSN

1687-8760

e-ISSN

—

Svazek periodika

2017

Číslo periodika v rámci svazku

"Article Number: 3712384"

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

"neuvedeno"

Kód UT WoS článku

000395147100001

EID výsledku v databázi Scopus

2-s2.0-85014422581