Superficially bound acetylcholinesterase based on a chitosan matrix for neurotoxiccCompound assay by a photographic technique

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889485" target="_blank" >RIV/60162694:G44__/18:43889485 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/00032719.2017.1381846?journalCode=lanl20" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/00032719.2017.1381846?journalCode=lanl20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00032719.2017.1381846" target="_blank" >10.1080/00032719.2017.1381846</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Superficially bound acetylcholinesterase based on a chitosan matrix for neurotoxiccCompound assay by a photographic technique

Popis výsledku v původním jazyce

Smartphones have become popular in the last decade and they have been used in analytical chemistry as easy detection systems with comparable parameters to standard laboratory equipment. This work focuses on the attachment of enzyme acetylcholinesterase on the previously activated chitosan. Acetylcholine splits the neurotransmitter acetylcholine, as a natural substrate, into choline and acetic acid. However, this compound can cleave alternative substrates, e.g., indoxyl acetate, which was used in this work. The conversion of the substrate is blocked or slowed down by inhibitors from which galantamine and tacrine were tested as model inhibitors with detection limits of 1.1 and 0.18 mu M, respectively. The measurement procedure was performed on a three-dimensional printed holder and red-green-blue channels were used for digital photography evaluation. The method was validated using a standard Ellman&apos;s spectrophotometric assay. We successfully attached acetylcholinesterase on the chitosan surface that was used for the inhibitor assay. The long-term stability of the immobilized enzyme as well as the sensitivity to organic solvents were also tested. The proposed method appeared to be suitable for the rapid and inexpensive assay of neurotoxic compounds.

Název v anglickém jazyce

Superficially bound acetylcholinesterase based on a chitosan matrix for neurotoxiccCompound assay by a photographic technique

Popis výsledku anglicky

Smartphones have become popular in the last decade and they have been used in analytical chemistry as easy detection systems with comparable parameters to standard laboratory equipment. This work focuses on the attachment of enzyme acetylcholinesterase on the previously activated chitosan. Acetylcholine splits the neurotransmitter acetylcholine, as a natural substrate, into choline and acetic acid. However, this compound can cleave alternative substrates, e.g., indoxyl acetate, which was used in this work. The conversion of the substrate is blocked or slowed down by inhibitors from which galantamine and tacrine were tested as model inhibitors with detection limits of 1.1 and 0.18 mu M, respectively. The measurement procedure was performed on a three-dimensional printed holder and red-green-blue channels were used for digital photography evaluation. The method was validated using a standard Ellman&apos;s spectrophotometric assay. We successfully attached acetylcholinesterase on the chitosan surface that was used for the inhibitor assay. The long-term stability of the immobilized enzyme as well as the sensitivity to organic solvents were also tested. The proposed method appeared to be suitable for the rapid and inexpensive assay of neurotoxic compounds.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Letters

ISSN

0003-2719

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1622-1632

Kód UT WoS článku

000429348300013

EID výsledku v databázi Scopus

2-s2.0-85044085562