Targeted mass spectrometry analysis of Clostridium perfringens toxins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F19%3A00537016" target="_blank" >RIV/60162694:G44__/19:00537016 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G33__/19:N0000006 RIV/62690094:18470/19:50015557

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468457/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6468457/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/toxins11030177" target="_blank" >10.3390/toxins11030177</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Targeted mass spectrometry analysis of Clostridium perfringens toxins

Popis výsledku v původním jazyce

Targeted proteomics recently proved to be a technique for the detection and absolute quantification of proteins not easily accessible to classical bottom-up approaches. Due to this, it has been considered as a high fidelity tool to detect potential warfare agents in wide spread kinds of biological and environmental matrices. Clostridium perfringens toxins are considered to be potential biological weapons, especially the epsilon toxin which belongs to a group of the most powerful bacterial toxins. Here, the development of a target mass spectrometry method for the detection of C. perfringens protein toxins (alpha, beta, beta2, epsilon, iota) is described. A high-resolution mass spectrometer with a quadrupole-Orbitrap system operating in target acquisition mode (parallel reaction monitoring) was utilized. Because of the lack of commercial protein toxin standards recombinant toxins were prepared within Escherichia coli. The analysis was performed using proteotypic peptides as the target compounds together with their isotopically labeled synthetic analogues as internal standards. Calibration curves were calculated for each peptide in concentrations ranging from 0.635 to 1101 fmol/mu L. Limits of detection and quantification were determined for each peptide in blank matrices.

Název v anglickém jazyce

Targeted mass spectrometry analysis of Clostridium perfringens toxins

Popis výsledku anglicky

Targeted proteomics recently proved to be a technique for the detection and absolute quantification of proteins not easily accessible to classical bottom-up approaches. Due to this, it has been considered as a high fidelity tool to detect potential warfare agents in wide spread kinds of biological and environmental matrices. Clostridium perfringens toxins are considered to be potential biological weapons, especially the epsilon toxin which belongs to a group of the most powerful bacterial toxins. Here, the development of a target mass spectrometry method for the detection of C. perfringens protein toxins (alpha, beta, beta2, epsilon, iota) is described. A high-resolution mass spectrometer with a quadrupole-Orbitrap system operating in target acquisition mode (parallel reaction monitoring) was utilized. Because of the lack of commercial protein toxin standards recombinant toxins were prepared within Escherichia coli. The analysis was performed using proteotypic peptides as the target compounds together with their isotopically labeled synthetic analogues as internal standards. Calibration curves were calculated for each peptide in concentrations ranging from 0.635 to 1101 fmol/mu L. Limits of detection and quantification were determined for each peptide in blank matrices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

<a href="/cs/project/VH20172020012" target="_blank" >VH20172020012: Příprava kolekce standardů biologicky významných toxinů s podporou Evropské sitě laboratoiří biologické ochrany (European biodefence laboratory network)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxins

ISSN

2072-6651

e-ISSN

2072-6651

Svazek periodika

11

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

177

Kód UT WoS článku

000464472400001

EID výsledku v databázi Scopus

2-s2.0-85063712474