Simple detection of primary cilia by immunofluorescence
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555795" target="_blank" >RIV/60162694:G44__/20:00555795 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11110/20:10425140 RIV/00216208:11150/20:10425140 RIV/00179906:_____/20:10425140 RIV/00064190:_____/20:N0000070
Výsledek na webu
<a href="https://www.jove.com/video/61155/simple-detection-of-primary-cilia-by-immunofluorescence" target="_blank" >https://www.jove.com/video/61155/simple-detection-of-primary-cilia-by-immunofluorescence</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/61155" target="_blank" >10.3791/61155</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Simple detection of primary cilia by immunofluorescence
Popis výsledku v původním jazyce
Primary cilia are dynamically regulated during cell cycle progression, specifically during the G0/G1 phases of the cell cycle, being resorbed prior to mitosis. Primary cilia can be visualized with highly sophisticated methods, including transmission electron microscopy, 3D imaging, or using software for the automatic detection of primary cilia. However, immunofluorescent staining of primary cilia is needed to perform these methods. This publication describes a protocol for the easy detection of primary cilia in vitro by staining acetylated alpha tubulin (axoneme) and gamma tubulin (basal body). This immunofluorescent staining protocol is relatively simple and results in high-quality images. The present protocol describes how four cell lines (C2C12, MEF, NHLF, and skin fibroblasts) expressing primary cilia were fixed, immunostained, and imaged with a fluorescent or confocal microscope.
Název v anglickém jazyce
Simple detection of primary cilia by immunofluorescence
Popis výsledku anglicky
Primary cilia are dynamically regulated during cell cycle progression, specifically during the G0/G1 phases of the cell cycle, being resorbed prior to mitosis. Primary cilia can be visualized with highly sophisticated methods, including transmission electron microscopy, 3D imaging, or using software for the automatic detection of primary cilia. However, immunofluorescent staining of primary cilia is needed to perform these methods. This publication describes a protocol for the easy detection of primary cilia in vitro by staining acetylated alpha tubulin (axoneme) and gamma tubulin (basal body). This immunofluorescent staining protocol is relatively simple and results in high-quality images. The present protocol describes how four cell lines (C2C12, MEF, NHLF, and skin fibroblasts) expressing primary cilia were fixed, immunostained, and imaged with a fluorescent or confocal microscope.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10700 - Other natural sciences
Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
1940-087X
Svazek periodika
2020
Číslo periodika v rámci svazku
159
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
e61155
Kód UT WoS článku
000546406600049
EID výsledku v databázi Scopus
2-s2.0-85085362508