Study of the latent phase of radiation-induced lung injury

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00556003" target="_blank" >RIV/60162694:G44__/20:00556003 - isvavai.cz</a>

Výsledek na webu

<a href="https://mmsl.cz/corproof.php?tartkey=mms-000000-0130" target="_blank" >https://mmsl.cz/corproof.php?tartkey=mms-000000-0130</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.31482/mmsl.2020.015" target="_blank" >10.31482/mmsl.2020.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of the latent phase of radiation-induced lung injury

Popis výsledku v původním jazyce

Early changes in lung tissue after radiation exposure may serve as biodosimetric markers as well as targets for mitigation of radiation-induced lung injury. The aim of our study was to examine cytokine, cellular and tissue alterations induced by ionizing radiation during the first month after irradiation. Female C57BL/6 mice were total body irradiated (TBI) by 8 Gy. Samples of blood and lung tissue were collected 4, 8 and 24 h, 7, 21 and 30 d after irradiation. Blood count, immunophenotypisation and cytokine profile were measured in blood. In lung, immunophenotypisation, cytokine profile and histopathology were evaluated. In blood, the acute radiation syndrome developed with recovery being observed at 21 – 30 d. In the lung tissue, a biphasic response occurred. At first, a significant decreased of lymphocytes, resident tissues macrophages and air/tissue ratio associated with increased neutrophils were observed at 8 – 24 h. Subsequently, increase in infiltrating CD4+ T-lymphocytes, neutrophils and resident tissues macrophages and decreased airiness were measured 21 and 30 d after TBI. In summary, our study describes the mechanisms that lung tissue enables to cope with non-lethal injury.

Název v anglickém jazyce

Study of the latent phase of radiation-induced lung injury

Popis výsledku anglicky

Early changes in lung tissue after radiation exposure may serve as biodosimetric markers as well as targets for mitigation of radiation-induced lung injury. The aim of our study was to examine cytokine, cellular and tissue alterations induced by ionizing radiation during the first month after irradiation. Female C57BL/6 mice were total body irradiated (TBI) by 8 Gy. Samples of blood and lung tissue were collected 4, 8 and 24 h, 7, 21 and 30 d after irradiation. Blood count, immunophenotypisation and cytokine profile were measured in blood. In lung, immunophenotypisation, cytokine profile and histopathology were evaluated. In blood, the acute radiation syndrome developed with recovery being observed at 21 – 30 d. In the lung tissue, a biphasic response occurred. At first, a significant decreased of lymphocytes, resident tissues macrophages and air/tissue ratio associated with increased neutrophils were observed at 8 – 24 h. Subsequently, increase in infiltrating CD4+ T-lymphocytes, neutrophils and resident tissues macrophages and decreased airiness were measured 21 and 30 d after TBI. In summary, our study describes the mechanisms that lung tissue enables to cope with non-lethal injury.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Military Medical Science Letters

ISSN

0372-7025

e-ISSN

2571-113X

Svazek periodika

89

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

190-199

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85097416112