A colour sensor integrated into a microcontroller platform as a reliable tool for measuring pH changes in biochemistry applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F25%3A00563653" target="_blank" >RIV/60162694:G44__/25:00563653 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00637b" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2024/ay/d4ay00637b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d4ay00637b" target="_blank" >10.1039/d4ay00637b</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A colour sensor integrated into a microcontroller platform as a reliable tool for measuring pH changes in biochemistry applications

Popis výsledku v původním jazyce

Colorimetry is a widely used technique for optical detection in point-of-care testing and on-site detection. Although some studies employ a multiplex approach to analyse coloured solutions, many still analyse one sample at a time. We have prepared a simple and affordable colorimetric assay based on a TCS34725 colour sensor (ams-OSRAM) integrated into an M5Stack module and an RGB LED module both inserted into a 3D printed frame. We found that the colorimetric assay can be easily transferred to a colour sensing platform, and the signal range obtained using the prepared colorimeter is more than 200 times larger than that obtained using digital image colorimetry (DIC) for the same samples containing cholinesterase or urease as a model enzyme providing a change in pH of the processed solution. The assay appears to be ready for practical use. An open-source colorimeter with a colour sensor for determination of pH in liquids with a universal indicator - a novel approach for reaction monitoring.

Název v anglickém jazyce

A colour sensor integrated into a microcontroller platform as a reliable tool for measuring pH changes in biochemistry applications

Popis výsledku anglicky

Colorimetry is a widely used technique for optical detection in point-of-care testing and on-site detection. Although some studies employ a multiplex approach to analyse coloured solutions, many still analyse one sample at a time. We have prepared a simple and affordable colorimetric assay based on a TCS34725 colour sensor (ams-OSRAM) integrated into an M5Stack module and an RGB LED module both inserted into a 3D printed frame. We found that the colorimetric assay can be easily transferred to a colour sensing platform, and the signal range obtained using the prepared colorimeter is more than 200 times larger than that obtained using digital image colorimetry (DIC) for the same samples containing cholinesterase or urease as a model enzyme providing a change in pH of the processed solution. The assay appears to be ready for practical use. An open-source colorimeter with a colour sensor for determination of pH in liquids with a universal indicator - a novel approach for reaction monitoring.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

1759-9679

Svazek periodika

16

Číslo periodika v rámci svazku

38

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

6487-6493

Kód UT WoS článku

001304076300001

EID výsledku v databázi Scopus

2-s2.0-85203004328