Application of dicentric chromosome assay for evaluation of radioprotective effect

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F26%3A00564806" target="_blank" >RIV/60162694:G44__/26:00564806 - isvavai.cz</a>

Výsledek na webu

<a href="https://academic.oup.com/biomethods/article/10/1/bpaf058/8222721?login=true" target="_blank" >https://academic.oup.com/biomethods/article/10/1/bpaf058/8222721?login=true</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/biomethods/bpaf058" target="_blank" >10.1093/biomethods/bpaf058</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of dicentric chromosome assay for evaluation of radioprotective effect

Popis výsledku v původním jazyce

The dicentric chromosome assay is a well-established biodosimetric method used to assess absorbed ionizing radiation doses by detecting dicentric chromosomal aberrations. Here, we present a detailed, reproducible protocol for applying the dicentric chromosome assay for in vitro evaluation of radioprotective agents, including novel piperazine derivatives compared with amifostine and its active metabolite WR-1065. The protocol covers all key steps-blood sample preparation, in vitro irradiation, lymphocyte culture, metaphase preparation, and scoring of dicentric chromosomes. It highlights critical stages that affect data quality and reproducibility. Integrating manual scoring with automated analysis using the Metafer system ensures accurate and efficient assessment. Thus, this protocol bridges the fields of biological dosimetry and preclinical screening of radioprotective agents, providing a reliable framework for emergency radiation dose estimation and the development of new radiation medical countermeasures.

Název v anglickém jazyce

Application of dicentric chromosome assay for evaluation of radioprotective effect

Popis výsledku anglicky

The dicentric chromosome assay is a well-established biodosimetric method used to assess absorbed ionizing radiation doses by detecting dicentric chromosomal aberrations. Here, we present a detailed, reproducible protocol for applying the dicentric chromosome assay for in vitro evaluation of radioprotective agents, including novel piperazine derivatives compared with amifostine and its active metabolite WR-1065. The protocol covers all key steps-blood sample preparation, in vitro irradiation, lymphocyte culture, metaphase preparation, and scoring of dicentric chromosomes. It highlights critical stages that affect data quality and reproducibility. Integrating manual scoring with automated analysis using the Metafer system ensures accurate and efficient assessment. Thus, this protocol bridges the fields of biological dosimetry and preclinical screening of radioprotective agents, providing a reliable framework for emergency radiation dose estimation and the development of new radiation medical countermeasures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/NU23-08-00256" target="_blank" >NU23-08-00256: Hit-to-lead vývoj malých molekul pro zlepšení radiační ochrany v terapii a diagnostice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology Methods & Protocols

ISSN

—

e-ISSN

2396-8923

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

bpaf058

Kód UT WoS článku

001548852200001

EID výsledku v databázi Scopus

2-s2.0-105014780638