Experimental adhesion of Geobacillus stearothermophilus and Anoxybacillus flavithermus to stainless steel compared with predictions from interaction models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60193697%3A_____%2F20%3AN0000011" target="_blank" >RIV/60193697:_____/20:N0000011 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11696-019-00880-0" target="_blank" >https://link.springer.com/article/10.1007/s11696-019-00880-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-019-00880-0" target="_blank" >10.1007/s11696-019-00880-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Experimental adhesion of Geobacillus stearothermophilus and Anoxybacillus flavithermus to stainless steel compared with predictions from interaction models

Popis výsledku v původním jazyce

Spore-forming thermophilic bacteria of the genus Geobacillus and Anoxybacillus are frequent contaminants in dairy industry. This study is the first attempt to apply models of physicochemical interactions (thermodynamic, DLVO, and XDLVO) to quantify their adhesion properties to stainless steel particles (SSP). The predictions of interaction models were compared with experimental data (contact angles, zeta potentials, size) regarding interacting surfaces (cells and SSP). Adhesion intensities (AI) were determined experimentally taking advantage of the magnetic properties of particulate stainless steel. The importance of weak physicochemical interactions was estimated by comparison of experimental AI with model predictions of colloidal interactions. The results revealed that the most reliable description of AI was obtained using the XDLVO model, including Lifshitz–van der Waals (LW), acid–base, and electrostatic (EL) interactions. The AI of cells to SSP at an ionic strength of 10 mM decreased in the order G. stearothermophilus DSM 456 > A. flavithermus DSM 2641 > G. stearothermophilus DSM 22, and the differences were statistically significant. At a higher ionic strength (100 mM), the highest AI was observed for A. flavithermus DSM 2641, but the differences between species studied were statistically insignificant. The main driving force for bacterial adhesion to SSP at 10 mM was EL interactions, while at 100 mM, the XDLVO model predicted favorable interactions between A. flavithermus DSM 2641 and SSP due to attractive LW forces.

Název v anglickém jazyce

Experimental adhesion of Geobacillus stearothermophilus and Anoxybacillus flavithermus to stainless steel compared with predictions from interaction models

Popis výsledku anglicky

Spore-forming thermophilic bacteria of the genus Geobacillus and Anoxybacillus are frequent contaminants in dairy industry. This study is the first attempt to apply models of physicochemical interactions (thermodynamic, DLVO, and XDLVO) to quantify their adhesion properties to stainless steel particles (SSP). The predictions of interaction models were compared with experimental data (contact angles, zeta potentials, size) regarding interacting surfaces (cells and SSP). Adhesion intensities (AI) were determined experimentally taking advantage of the magnetic properties of particulate stainless steel. The importance of weak physicochemical interactions was estimated by comparison of experimental AI with model predictions of colloidal interactions. The results revealed that the most reliable description of AI was obtained using the XDLVO model, including Lifshitz–van der Waals (LW), acid–base, and electrostatic (EL) interactions. The AI of cells to SSP at an ionic strength of 10 mM decreased in the order G. stearothermophilus DSM 456 > A. flavithermus DSM 2641 > G. stearothermophilus DSM 22, and the differences were statistically significant. At a higher ionic strength (100 mM), the highest AI was observed for A. flavithermus DSM 2641, but the differences between species studied were statistically insignificant. The main driving force for bacterial adhesion to SSP at 10 mM was EL interactions, while at 100 mM, the XDLVO model predicted favorable interactions between A. flavithermus DSM 2641 and SSP due to attractive LW forces.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

2585-7290

e-ISSN

1336-9075

Svazek periodika

74

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

297-304

Kód UT WoS článku

000510432900023

EID výsledku v databázi Scopus

2-s2.0-85069510500