Occurrence of cytochalasin E in malting barley and malt in the Czech Republic

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of cytochalasin E in malting barley and malt in the Czech Republic

Popis výsledku v původním jazyce

The microscopic fungus Aspergillus clavatus hardly occurs as a field contaminant of plants. It belongs to storage fungi, when it was found in a number of products stored in unsuitable conditions with high humidity. Its growth is mainly associated with increased temperatures during the malting of barley and other cereals [1]. Cytochalasin E is one of the toxic secondary metabolites produced by Aspergillus clavatus [2]. It is an inhibitor of actin polymerization in platelets. It also damages blood capillary cells, allows extravascular diffusion of plasma fluid, and prevents angiogenesis (regeneration of blood capillaries). It has mainly cytotoxic effects.Cytochalasin E was analyzed in Czech malting barley and malt. For the extraction, the QuEChERS method was used and the mycotoxin was identified and quantified using ultra performance liquid chromatography with mass spectrometric detection. A total of 598 barley and malt samples were analyzed. In total, almost 1% of barley samples and 21% of malt samples were contaminated. The concentration of cytochalasin E in barley ranged from 13.5 µg/kg to 52.2 µg/kg with mean being 23.3 µg/kg and in malt ranged from 0.6 µg/kg to 135.2 µg/kg with mean being 25.1 µg/kg. Results were presented in the form of a poster at the conference 44. Mycotoxin Workshop, Hannover, Germany, 5-7 June 2023.

Název v anglickém jazyce

Occurrence of cytochalasin E in malting barley and malt in the Czech Republic

Popis výsledku anglicky

The microscopic fungus Aspergillus clavatus hardly occurs as a field contaminant of plants. It belongs to storage fungi, when it was found in a number of products stored in unsuitable conditions with high humidity. Its growth is mainly associated with increased temperatures during the malting of barley and other cereals [1]. Cytochalasin E is one of the toxic secondary metabolites produced by Aspergillus clavatus [2]. It is an inhibitor of actin polymerization in platelets. It also damages blood capillary cells, allows extravascular diffusion of plasma fluid, and prevents angiogenesis (regeneration of blood capillaries). It has mainly cytotoxic effects.Cytochalasin E was analyzed in Czech malting barley and malt. For the extraction, the QuEChERS method was used and the mycotoxin was identified and quantified using ultra performance liquid chromatography with mass spectrometric detection. A total of 598 barley and malt samples were analyzed. In total, almost 1% of barley samples and 21% of malt samples were contaminated. The concentration of cytochalasin E in barley ranged from 13.5 µg/kg to 52.2 µg/kg with mean being 23.3 µg/kg and in malt ranged from 0.6 µg/kg to 135.2 µg/kg with mean being 25.1 µg/kg. Results were presented in the form of a poster at the conference 44. Mycotoxin Workshop, Hannover, Germany, 5-7 June 2023.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů