Detection of Resistance Genes in Solanaceae Using DNA Markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F01%3A00002739" target="_blank" >RIV/60460709:41210/01:00002739 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Resistance Genes in Solanaceae Using DNA Markers

Popis výsledku v původním jazyce

As model botanic species were selected tomato (Lycopersicon esculentum) and potato (Solanum tuberosum ssp. tuberosum). The potato was assessed for the collection of tetraploidic gene sources. The tomato was assessed for gene sources - varieties of the F1generation type and the segregating F2 generation. The potato's resistance against Ro1 of the Globodera rostochiensis pathotype was marked with a PCR amplification of the Gro 1.2.3 loci in chromosome II.. To assess the resistance, the results of experiments conducted by Leister et al. (1997) were used. One loci of quantitative resistant - PCR marker has a close gene link with the monitored resistance. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. The experiments were based on tests by Williamson et al. (1994). Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR

Název v anglickém jazyce

Detection of Resistance Genes in Solanaceae Using DNA Markers

Popis výsledku anglicky

As model botanic species were selected tomato (Lycopersicon esculentum) and potato (Solanum tuberosum ssp. tuberosum). The potato was assessed for the collection of tetraploidic gene sources. The tomato was assessed for gene sources - varieties of the F1generation type and the segregating F2 generation. The potato's resistance against Ro1 of the Globodera rostochiensis pathotype was marked with a PCR amplification of the Gro 1.2.3 loci in chromosome II.. To assess the resistance, the results of experiments conducted by Leister et al. (1997) were used. One loci of quantitative resistant - PCR marker has a close gene link with the monitored resistance. For the dominantly established tomato's resistance against Meloidogyne incognita was used a co-dominant CAPS marker of the Mi gene. The experiments were based on tests by Williamson et al. (1994). Dominant and recessive alleles were detected after the digestion of the primary PCR product by the restriction enzyme TaqI. The identified PCR

Druh

D - Stať ve sborníku

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

4th International Symposium in the Series Recent Advances in Plant Biotechnology, Plant Molecular Biology for the New Millennium, Book of Abstracts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

47

Název nakladatele

Ústav molekulární biologie rostlin AV ČR

Místo vydání

České Budějovice

Místo konání akce

Třeboň

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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