Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F02%3A00004302" target="_blank" >RIV/60460709:41210/02:00004302 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:_____/02:00004302

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Popis výsledku v původním jazyce

Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Kašperské hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (

Název v anglickém jazyce

Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Popis výsledku anglicky

Actual methods of molecular analyses of DNA are routinely used at fields of zoological and botanical taxonomy, pest management and plant breeding. Knowledge of species-composition in populations of potato cyst nematodes (Globodera rostochiensis, G. pallida) is very important for selection of appropriate measure of regulation PCN's occurrence. The molecular method for distinguishing of PCN species is described at this article. There were used European populations of PCN - Šluknov (Ro1), Obersteinbach (Ro2), Harmerz (Ro5), Kalle (Pa2), Chavornay (Pa3), Delmsen (Pa3), and some cysts of unknown patotype from Kašperské hory (K) locality. Species-specific sets of primers for ITS-1 (Internal Transcribed Spacer 1) amplification were designed on base of known sequences ITS-1 of both PCN species by using of freeware Primers! for the World Wide Web. By using of set Fro1 - Rro1 was product 411bp detected (only in cause G. rostochiensis), by using of set Fpa2 - Rpa1 the product 239bp was detected (

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/EP9107" target="_blank" >EP9107: Analýza donorů rezistence k háďátku bramborovému v genofondu bramboru pomocí DNA markerů.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Rostlinná výroba (Plant Production)

ISSN

0370-663X

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

486-489

Kód UT WoS článku

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EID výsledku v databázi Scopus

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