Extended sequence analysis of three Danish Potato mop-top virus (PMTV)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A8066" target="_blank" >RIV/60460709:41210/04:8066 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Pokročilá analýza sekvencí 3 dánských Mop-top virů bramboru

Popis výsledku v původním jazyce

The entire nucleotide sequence for the coding regions of a Danish PMTV isolate 54-15 was determined and compared to other known and sequenced isolates of PMTV. Many nucleotide and amino acid changes were found in parts of RNA coding for the triple gene block (TGB) proteins and part of the RNA coding for the read-through region of the coat protein (CP). These regions for two other isolates, the mild one 54-10 and the severe one 54-19, were sequenced. Only two amino acid changes were found that correlatedwith the subdivision of isolates according to symptom development into mild and severe subgroups. Although the sequence comparisons indicate a high genetic stability of PMTV populations, a surprising change was found in the newly sequenced isolates - the replacement of the AUG start codon of the fourth gene of the TGB encoding RNA, coding for a cystein-rich protein, by the less efficient GUG start codon.

Název v anglickém jazyce

Pokročilá analýza sekvencí 3 dánských Mop-top virů bramboru

Popis výsledku anglicky

The entire nucleotide sequence for the coding regions of a Danish PMTV isolate 54-15 was determined and compared to other known and sequenced isolates of PMTV. Many nucleotide and amino acid changes were found in parts of RNA coding for the triple gene block (TGB) proteins and part of the RNA coding for the read-through region of the coat protein (CP). These regions for two other isolates, the mild one 54-10 and the severe one 54-19, were sequenced. Only two amino acid changes were found that correlatedwith the subdivision of isolates according to symptom development into mild and severe subgroups. Although the sequence comparisons indicate a high genetic stability of PMTV populations, a surprising change was found in the newly sequenced isolates - the replacement of the AUG start codon of the fourth gene of the TGB encoding RNA, coding for a cystein-rich protein, by the less efficient GUG start codon.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virus Genes

ISSN

0920-8569

e-ISSN

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Svazek periodika

2

Číslo periodika v rámci svazku

29

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

249-255

Kód UT WoS článku

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EID výsledku v databázi Scopus

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