Kadmium způsobuje poškození DNA v kořenech tabáku, ale neovlivňuje DNA, somatické mutace a homologické rekombinace v listech tabáku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F04%3A9190" target="_blank" >RIV/60460709:41210/04:9190 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/04:00107463

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves

Popis výsledku v původním jazyce

The heavy metal cadmium (Cd2+) applied on tobacco roots in the form of cadmium chloride, induced significantly higher levels of DNA damage as measured by the cellular Comet assay than did treatment of isolated root nuclei, analyzed by use of the acellular Comet assay. DNA damage induced by Cd2+ in roots of a transgenic catalase-deficient tobacco line (CAT1AS) was higher than in wild-type tobacco (SR1) roots. In contrast to treatment with the positive control ethyl methanesulphonate, Cd2+ induced no significant DNA damage in leaf nuclei, and neither somatic mutations, nor homologous recombination as measured by the GUS genereactivation assay, were observed in leaves. Analysis of the accumulation of cadmium by inductively coupled plasma optical emissionspectrometry demonstrates that roots accumulate almost 50-fold more cadmium than above-ground parts of the tobacco seedlings. This may explain the absence of Cd2+ genotoxicity in leaves.

Název v anglickém jazyce

Cadmium induces DNA damage in tobacco roots, but no DNA damage, somatic mutations or homologous recombination in tobacco leaves

Popis výsledku anglicky

The heavy metal cadmium (Cd2+) applied on tobacco roots in the form of cadmium chloride, induced significantly higher levels of DNA damage as measured by the cellular Comet assay than did treatment of isolated root nuclei, analyzed by use of the acellular Comet assay. DNA damage induced by Cd2+ in roots of a transgenic catalase-deficient tobacco line (CAT1AS) was higher than in wild-type tobacco (SR1) roots. In contrast to treatment with the positive control ethyl methanesulphonate, Cd2+ induced no significant DNA damage in leaf nuclei, and neither somatic mutations, nor homologous recombination as measured by the GUS genereactivation assay, were observed in leaves. Analysis of the accumulation of cadmium by inductively coupled plasma optical emissionspectrometry demonstrates that roots accumulate almost 50-fold more cadmium than above-ground parts of the tobacco seedlings. This may explain the absence of Cd2+ genotoxicity in leaves.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

ISSN

1383-5718

e-ISSN

—

Svazek periodika

559

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

49-57

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—