Polyklonální protilátky pro detekci roztoče moučného Acarus siro (Acari: Acaridida

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11974" target="_blank" >RIV/60460709:41210/05:11974 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/04:2807

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polyclonal antibodies for detection of flour mite Acarus siro (Acari: Acaridida

Popis výsledku v původním jazyce

Acaroid mites are serious pests of stored products and households.They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA.The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigenin the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated.We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, the rearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (ant

Název v anglickém jazyce

Polyclonal antibodies for detection of flour mite Acarus siro (Acari: Acaridida

Popis výsledku anglicky

Acaroid mites are serious pests of stored products and households.They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA.The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigenin the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated.We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, the rearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (ant

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QF4071" target="_blank" >QF4071: Komplexní metoda monitorování a detekce kontaminace skladovaných obilovin a cereálních produktů škůdci pomocí fyzikálně-chemických, imunochemických a molekulárních technik.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Phytophaga

ISSN

0393-8131

e-ISSN

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Svazek periodika

2004

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

10

Strana od-do

711-720

Kód UT WoS článku

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EID výsledku v databázi Scopus

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